Simultaneous fluorescence imaging of selenol and hydrogen peroxide under normoxia and hypoxia in HepG2 cells and in vivo
Simultaneous fluorescence imaging of selenol and hydrogen peroxide under normoxia and hypoxia in HepG2 cells and in vivo
复制标题
HepG2细胞和体内常氧和缺氧条件下硒醇和过氧化氢的同步荧光成像
DOI：
10.1039/c6cc02111e
复制
发表时间：
2016-01-01
影响因子：
4.9
通讯作者：
Tang, Bo
中科院分区：
文献类型：
--
作者：
Liu, Xiaojun;Hu, Bo;Tang, Bo
基于硒醇对Au-S键的快速取代反应，我们设计并合成了硒醇的5-FAM-peptide-AuNPs纳米探针。实时成像结果表明，该探针与分子探针QCy7-H2O2能够同时、差异地监测活细胞和体内硒醇和H2O2的浓度。
Based on the rapid substitution reaction of the Au-S bond by selenol, we designed and synthesized a nanoprobe 5-FAM-peptide-AuNPs for selenol. Real-time imaging shows that this probe together with the molecular probe QCy7-H2O2 is able to simultaneously and differentially monitor the concentrations of selenol and H2O2 in living cells and in vivo.