Differential Adhesive Properties of Sequestered Asexual and Sexual Stages of Plasmodium falciparum on Human Endothelial Cells Are Tissue Independent
Differential Adhesive Properties of Sequestered Asexual and Sexual Stages of Plasmodium falciparum on Human Endothelial Cells Are Tissue Independent
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DOI：
10.1371/journal.pone.0031567
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发表时间：
2012-02-21
期刊：
影响因子：
3.7
通讯作者：
Alano, Pietro
中科院分区：
文献类型：
--
作者：
Silvestrini, Francesco;Tiburcio, Marta;Alano, Pietro
原生动物寄生虫恶性疟原虫是最严重的疟疾，在感染过程中能够与外周循环隔离。无性阶段的寄生虫通过与各种器官的微血管系统中的内皮细胞受体结合来隔离。恶性疟原虫配子体是负责从人类向按蚊传播寄生虫的发育阶段，在它们被释放到血液主流中之前，它们的成熟也花费了几乎十天的时间远离外周循环。与无性寄生虫相反，负责未成熟配子体隔离的机制和细胞相互作用在很大程度上未被探索，迄今为止已经产生了关于这个问题的有争议的证据。在这里，我们提出了一个系统的比较无性阶段和不成熟和成熟的配子母细胞的参考恶性疟原虫克隆3D 7和病人的寄生虫分离的人内皮细胞从不同组织的面板上的细胞结合特性。该分析包括对人骨髓源性内皮细胞系（HBMEC）的测定，因为该组织被认为是配子母细胞成熟的主要部位。我们的研究结果清楚地表明，细胞粘附的无性阶段的寄生虫是一贯更有效的比，几乎检测不到的未成熟的配子体，无论使用的内皮细胞系和寄生虫的基因型。重要的是，与骨髓来源的内皮细胞上的无性阶段相比，本文测试的两种细胞系的未成熟配子母细胞未显示出更高的结合效率，这与先前在关于该问题的唯一研究中报道的不同。这表明，在这个组织中的配子-宿主相互作用是不太可能介导的相同的粘附过程中看到的特异性内皮受体的无性形式。
The protozoan parasite Plasmodium falciparum, responsible for the most severe form of malaria, is able to sequester from peripheral circulation during infection. The asexual stage parasites sequester by binding to endothelial cell receptors in the microvasculature of various organs. P. falciparum gametocytes, the developmental stages responsible for parasite transmission from humans to Anopheles mosquitoes, also spend the almost ten days necessary for their maturation sequestered away from the peripheral circulation before they are released in blood mainstream. In contrast to those of asexual parasites, the mechanisms and cellular interactions responsible for immature gametocyte sequestration are largely unexplored, and controversial evidence has been produced so far on this matter. Here we present a systematic comparison of cell binding properties of asexual stages and immature and mature gametocytes from the reference P. falciparum clone 3D7 and from a patient parasite isolate on a panel of human endothelial cells from different tissues. This analysis includes assays on human bone marrow derived endothelial cell lines (HBMEC), as this tissue has been proposed as a major site of gametocyte maturation. Our results clearly demonstrate that cell adhesion of asexual stage parasites is consistently more efficient than that, virtually undetectable of immature gametocytes, irrespectively of the endothelial cell lines used and of parasite genotypes. Importantly, immature gametocytes of both lines tested here do not show a higher binding efficiency compared to asexual stages on bone marrow derived endothelial cells, unlike previously reported in the only study on this issue. This indicates that gametocyte-host interactions in this tissue are unlikely to be mediated by the same adhesion processes to specific endothelial receptors as seen with asexual forms.