Prokaryotic expression and purification of HA1 and HA2 polypeptides for serological analysis of the 2009 pandemic H1N1 influenza virus
Prokaryotic expression and purification of HA1 and HA2 polypeptides for serological analysis of the 2009 pandemic H1N1 influenza virus
复制标题
用于2009年大流行H1N1流感病毒血清学分析的HA1和HA2多肽的原核表达和纯化
DOI：
10.1016/j.jviromet.2010.12.007
复制
发表时间：
2011-03-01
影响因子：
3.1
通讯作者：
Tong, Yigang
中科院分区：
文献类型：
--
作者：
Liu, Dabin;Si, Bingyin;Tong, Yigang
血凝素(HA)是一种重要的流感病毒表面抗原，在流感研究中占有重要地位。本研究将2009年大流行性流感病毒H1N1(A/California/04/2009(H1N1))的HA1和HA2蛋白编码基因克隆到原核表达载体pCodtf中，转化大肠杆菌，获得含有H1N1 HA1和HA2的可溶性融合蛋白。用抗A/California/04/2009(H1N1)全毒株的多克隆抗体和单抗对重组蛋白进行免疫印迹分析。重组蛋白的免疫反应性还通过人血清标本的酶联免疫吸附试验和血凝抑制试验进行了定性。这些结果将有助于未来对2009年大流行H1N1病毒HA的免疫学和血清学研究。(C)2010爱思唯尔B.V.保留所有权利。
Hemagglutinin (HA) is an important influenza virus surface antigen that is highly topical in influenza research. In the present study, the genes encoding the HA1 and HA2 proteins from the 2009 pandemic influenza virus H1N1 (A/California/04/2009(H1N1)) were cloned into a prokaryotic expression plasmid pCold-TF, and soluble fusion proteins containing H1N1 HA1 and HA2 were produced by transformed Escherichia coli. Western blot assays were used to examine the immunoreactivity of the recombinant proteins using polyclonal and monoclonal antibodies derived against the whole virus A/California/04/2009(H1N1). Recombinant protein immunoreactivity was also analyzed qualitatively by ELISA and hemagglutination inhibition using human serum samples. These results will aid future immunological and serological studies of the 2009 pandemic H1N1 virus HA. (C) 2010 Elsevier B.V. All rights reserved.