Study on chemical constituents and alpha-glucosidase inhibitory activity of Cyclocarya paliurus in Guizhou Province.
Study on chemical constituents and alpha-glucosidase inhibitory activity of Cyclocarya paliurus in Guizhou Province.
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DOI：
10.16333/j.1001-6880.2019.6.003
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Gu, W.
中科院分区：
文献类型：
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作者：
Duan, Yu-Shu;Hu, Yong;Gu, W.
为了阐明青钱柳的降血糖成分，对其化学成分及α - 葡萄糖苷酶抑制活性进行了系统研究。采用多种色谱方法和光谱方法从青钱柳树叶的80%乙醇提取物中分离并鉴定出13种化合物。这些化合物分别被鉴定为：山柰酚 - 3 - O -（4″ - O - 乙酰基） - α - L - 鼠李吡喃糖苷（1）、山柰酚3 - O -（4″ - O - 乙酰基） - α - L - 鼠李吡喃糖苷（2）、胡桃苷J（3）、1α, 2α, 4β - 三醇 - 1, 2, 3, 4 - 四氢萘（4）、青钱柳苷III（5）、青钱柳苷J（6）、孕酮（7）、12 - O - 去乙酰基美商陆皂苷元（8）、20α - 乙酰氧基孕甾 - 4 - 烯 - 3 - 酮（9）、齐墩果酸（10）、5 - O - 对香豆酰基奎宁酸甲酯（11）、亚麻酸甲酯（12）、棕榈酸（13）。其中，化合物9为一种新的天然产物；化合物2 - 4、7、8、11和13首次从该植物中分离得到。采用对硝基苯基 - α - D - 吡喃葡萄糖苷（PNPG）法对α - 葡萄糖苷酶抑制活性进行筛选，结果表明化合物1和2具有较强的α - 葡萄糖苷酶抑制活性。这些结果表明黄酮类化合物是青钱柳植物的一类降血糖活性成分。
In order to elucidate the hypoglycemic components of C. paliurus, the chemical constituents and the inhibitory activity of alpha-glucosidase were systematic studied. 13 compounds were isolated and identified from 80% ethanol extract of C. paliurus leaves by means of various chromatographic methods and spectroscopic methods. These compounds were characterized as afzelin (1), Kaempferol 3-O-(4"-O-acetyl)-alpha-L-rhamnopyranoside (2), juglanoside J (3), 1alpha, 2alpha, 4beta-triol-1, 2, 3, 4-tetrahydronaphthalene (4), Cyclocarioside III (5), Cyclocarioside J (6), Progesterone (7), 12-O-deacetylmetaplexigenin (8), 20alpha-acetoxypregn-4-en-3-one (9), Oleanolic acid (10), 5-O-P-coumaroylquinic acid methyl ester (11), Methyl linolenate (12), Palmitic acid (13). Among them, compound 9 was a new natural product; compounds 2-4, 7, 8, 11, and 13 were isolated from this plant for the first time. The inhibitory activity of alpha-glucosidase was screened by PNPG method; the results showed that compounds 1 and 2 have strong alpha-glucosidase inhibitory activity. These results indicated that flavonoid is one kind of hypoglycemic active components of C. paliurus plant.