Comparison of Microbiological and Probiotic Characteristics of Lactobacilli Isolates from Dairy Food Products and Animal Rumen Contents.
Comparison of Microbiological and Probiotic Characteristics of Lactobacilli Isolates from Dairy Food Products and Animal Rumen Contents.
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DOI：
10.3390/microorganisms3020198
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发表时间：
2015-04-15
期刊：
影响因子：
4.5
通讯作者：
Hussain MA
中科院分区：
文献类型：
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作者：
Jose NM;Bunt CR;Hussain MA
乳酸菌被用于益生菌食品制剂，并作为家禽和牲畜的饲料添加剂，因为食用它们对健康有益。本研究的目的是评估和比较从新西兰的商业乳制品和动物瘤胃内容物中分离的十种乳酸菌菌株的益生菌潜力。菌株的遗传鉴定均属于乳杆菌属，其中乳杆菌属为罗伊氏乳杆菌、鼠李糖乳杆菌和植物乳杆菌。所有分离株均未表现出溶血行为。乳源分离株对低pH胁迫表现出较好的耐受性。另一方面，瘤胃分离株对胆汁盐表现出更高的耐受性。所有分离株均对氨基糖苷类抗生素耐药，但大多数对氨苄西林敏感。瘤胃源性分离菌对单核增生李斯特菌、产气肠杆菌和门斯顿沙门氏菌有较高的抑制作用。随着ph值的降低，所有分离株的细菌粘附性都有所增加。这项对乳酸菌分离株的筛选研究已经评估并确定了潜在的益生菌候选菌，以供进一步评估。
Lactobacilli are employed in probiotic food preparations and as feed additives in poultry and livestock, due to health benefits associated with their consumption. The objective of this study was to evaluate and compare the probiotic potential of ten lactobacilli strains isolated from commercial dairy food products and animal rumen contents in New Zealand. Genetic identification of the isolates revealed that all belonged to the genus Lactobacillus, specifically the species L. reuteri, L. rhamnosus and L. plantarum. All isolates did not show any haemolytic behaviour. Isolates of dairy origin showed better tolerance to low pH stress. On the other hand, rumen isolates exhibited a higher tolerance to presence of bile salts. All isolates exhibited resistance to aminoglycoside antibiotics, however most were sensitive to ampicillin. Isolates of rumen origin demonstrated a higher inhibitory effect on Listeria monocytogenes, Enterobacter aerogenes and Salmonella menston. Bacterial adherence of all isolates increased with a decrease in pH. This screening study on lactobacilli isolates has assessed and identified potential probiotic candidates for further evaluation.