Cochlear immunochemistry - a new technique based on gelatin embedding
Cochlear immunochemistry - a new technique based on gelatin embedding
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DOI：
10.1016/s0165-0270(03)00211-5
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发表时间：
2003-10-15
影响因子：
3
通讯作者：
Shepherd, RK
中科院分区：
文献类型：
--
作者：
Hurley, PA;Clarke, M;Shepherd, RK
免疫化学的组织学处理往往是良好的解剖分辨和抗原性保存之间的折衷。能够保存组织结构的技术总是需要高温和苛刻的化学物质，或者两者兼而有之。可能的结果是抗原性、酶活性和核酸完整性降低。我们已经修改了一种现有的包埋介质，用于在生理温度下工作的耳蜗，并且避免了变性剂和有机溶剂。组织抗原性最大化，解剖细节得到保护，这通常是两个相互排斥的目标。该方法简单、快速、透明，易于实现耳蜗声定位。它也可能适用于冻融切片过程中易变形的其他非均质结构的渗透。(C)2003爱思唯尔B.V.保留所有权利。
Histological processing of the cochlea for immunochemistry is often a compromise between good anatomical resolution and preservation of antigenicity. Techniques able to preserve tissue architecture invariably demand elevated temperatures and harsh chemicals or a combination of both. The likely result is reduced antigenicity, enzyme activity and nucleic acid integrity. We have modified an existing embedding medium for use in the cochlea that operates at physiological temperature and avoids denaturing agents and organic solvents. Tissue antigenicity is maximised and anatomical detail preserved, normally two mutually exclusive goals. The method is attractive because of its simplicity, speed and transparency for easy cochlear orientation. It is also likely to be adaptable for the infiltration of other heterogeneous structures prone to distortion during frozen sectioning. (C) 2003 Elsevier B.V. All rights reserved.