Targeted Imaging and Chemo-Phototherapy of Brain Cancer by a Multifunctional Drug Delivery System
Targeted Imaging and Chemo-Phototherapy of Brain Cancer by a Multifunctional Drug Delivery System
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多功能药物输送系统对脑癌的靶向成像和化疗光疗
DOI：
10.1002/mabi.201500091
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发表时间：
2015-11-01
影响因子：
4.6
通讯作者：
Zhang, Yun
中科院分区：
文献类型：
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作者：
Hao, Yongwei;Wang, Lei;Zhang, Yun
本研究的目的是通过脑靶向肽angiopep-2的表面修饰，开发具有同时向脑内递送吲哚菁绿色（ICG）和多西他赛（DTX）功能的多功能聚乳酸-共-乙交酯（PLGA）纳米粒，以实现近红外（NIR）成像下脑胶质瘤的联合化疗-光疗。ICG被选为近红外成像和光疗剂，DTX被用作化疗剂。ICG和DTX同时掺入PLGA纳米粒具有更高的稳定性。这些纳米颗粒进一步通过掺入纳米颗粒中的1，2-二硬脂酰基-sn-甘油基-3-磷酸乙醇胺-N-[甲氧基（聚乙二醇）-2000]-马来酰亚胺的外部马来酰亚胺基团用血管肽素-2修饰。Angiopep-2修饰的PLGA/DTX/ICG纳米粒（ANG/PLGA/DTX/ICG NPs）的近红外图像引导的化学-光疗不仅在体外高度诱导U87 MG细胞死亡，而且在体内有效地延长脑原位U87 MG胶质瘤异种移植荷瘤小鼠的寿命。因此，本研究表明ANG/PLGA/DTX/ICG NPs具有用于胶质瘤联合化疗和光疗的潜力。
The aim of this study was to develop multifunctional poly lactide-co-glycolide (PLGA) nanoparticles with the ability to simultaneously deliver indocyanine green (ICG) and docetaxel (DTX) to the brain by surface decoration with the brain-targeting peptide angiopep-2 to achieve combined chemo-phototherapy for glioma under near-infrared (NIR) imaging. ICG was selected as a near-infrared imaging and phototherapy agent and DTX was employed as a chemotherapeutic agent. ICG and DTX were simultaneously incorporated into PLGA nanoparticles with higher stability. These nanoparticles were further decorated with angiopep-2 via the outer maleimide group of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000]-maleinimide incorporated in the nanoparticles. The NIR image-guided chemo-phototherapy of the angiopep-2 modified PLGA/DTX/ICG nanoparticles (ANG/PLGA/DTX/ICG NPs) not only highly induced U87MG cell death in vitro, but also efficiently prolonged the life span of the brain orthotopic U87MG glioma xenograft-bearing mice in vivo. Thus, this study suggests that ANG/PLGA/DTX/ICG NPs have the potential for combinatorial chemotherapy and phototherapy for glioma.