Kupffer Cells: Important Participant of Hepatic Alveolar Echinococcosis
Kupffer Cells: Important Participant of Hepatic Alveolar Echinococcosis
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枯否细胞：肝泡包虫病的重要参与者。
DOI：
10.3389/fcimb.2020.00008
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发表时间：
2020-01-29
影响因子：
5.7
通讯作者：
Ma, Xiumin
中科院分区：
文献类型：
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作者：
Liu, Yumei;Tian, Fengming;Ma, Xiumin
目的：枯否细胞（Kupffer cell，KCs）是肝脏内的巨噬细胞，在生理和病理状态下对肝脏内环境的稳定起着重要的调节作用。本研究旨在探讨KCs的抗包虫作用及肝星状细胞（HSC）活化在肝泡状棘球蚴病（AE）肝纤维化进展中的作用。结果：封闭组H&E染色显示正常小叶结构破坏，病变周围可见炎性浸润; Masson染色显示汇管区附近可见明显的蓝色胶原纤维沉积。免疫组化结果显示，KCs表面标志物CD 68和CD 163、细胞因子iNOS和Arg-1在炎症灶附近呈阳性表达。qRT-PCR结果显示，KCs分泌的TNF-α、IL-10和TGF-β 1显著高于远距离组（P < 0.01）。值得注意的是，抗炎细胞因子的表达水平略高于促炎细胞因子。免疫组化和qRT-PCR结果均显示HSCs活化标志物、α-SMA和Desmin的表达均显著增加。结论：KCs具有抗包虫病的免疫保护作用和促进肝纤维修复的作用，对肝AE患者具有潜在的治疗价值。
Aims: Kupffer cells (KCs) are the liver-resident macrophages and play a leading role in the regulation of liver homeostasis in physiological conditions and in pathology. The study aims to investigate the anti-echinococcosis effect of KCs and the effects of hepatic stellate cells (HSCs) activation in the progression of liver fibrosis in hepatic alveolar echinococcosis (hepatic AE).Methods: Hematoxylin-eosin (H&E) and Masson staining were used to assess the pathological inflammatory changes and collagen deposition, respectively. Immunohistochemistry and qRT-PCR were used to detect the number of aggregates of KCs, the expression of cytokines and activation of HSCs.Results: In the close group, H&E staining showed that the normal lobular structure was destroyed and inflammatory infiltration around the lesion could be observed, and Masson staining showed that blue collagen fibers were clearly deposited near the portal area. IHC showed that KCs surface markers CD68 and CD163, cytokine iNOS and Arg-1 were positively expressed in the vicinity of inflammatory lesions. qRT-PCR indicated that TNF-alpha, IL-10, and TGF-beta 1 secreted by KCs were significantly higher than those in the distance group (P < 0.01). It is worth noticing that the expression levels of anti-inflammatory cytokines were slightly higher than that of pro-inflammatory cytokines. Both IHC and qRT-PCR results showed that HSCs activation markers, the expression of alpha-SMA and Desmin significantly increased.Conclusions: Our research indicates that KCs have immune-protective effect of anti-echinococcosis and promote liver fiber repair, and it also suggests that they have potential therapeutic value for patients with hepatic AE.