Antibiotic resistance of Vibrio harveyi isolated from seawater in Korea
Antibiotic resistance of Vibrio harveyi isolated from seawater in Korea
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DOI：
10.1016/j.marpolbul.2014.07.008
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发表时间：
2014-09-15
影响因子：
5.8
通讯作者：
So, Jae-Seong
中科院分区：
文献类型：
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作者：
Kang, Chang-Ho;Kim, YongGyeong;So, Jae-Seong
哈维氏弧菌是一种机会性人类病原体，可引起胃肠炎、严重坏死性软组织感染和原发性败血症，具有潜在的高致死率。在这项研究中，我们从韩国西海收集的海水中分离和鉴定了哈维氏弧菌，包括位于贝类养殖场附近的地点。对于推定的哈维氏弧菌的初始分离，将分离物在硫代硫酸盐柠檬酸盐胆盐蔗糖琼脂平板上孵育24小时，然后选择绿色菌落。随后通过生化试验和API 20 E试剂盒检测系统确认革兰氏阴性和氧化酶阳性菌落。根据哈氏弧菌的16S rRNA基因和溶血素基因设计特异性PCR引物。从840份海水样品中分离到2株哈维氏弧菌。这两个菌株进行了分析，对16种抗生素，并发现耐头孢噻吩，万古霉素，氨苄西林，头孢吡肟，头孢替坦，链霉素。(C)2014爱思唯尔有限公司版权所有。
Vibrio harveyi is an opportunistic human pathogen that may cause gastroenteritis, severe necrotizing soft-tissue infections, and primary septicemia, with a potentially high rate of lethality. In this study, we isolated and characterized V. harveyi from seawater collected from the West Sea in Korea, including sites located near shellfish farms. For the initial isolation of putative V. harveyi, isolates were incubated on thiosulfate citrate bile salt sucrose agar plates for 24 h, followed by selection of greenish colonies. Gram-negative and oxidase-positive colonies were subsequently confirmed by biochemical assays and the API 20E kit test system. Species-specific 16S rRNA and hemolysin genes were used to design V. harveyi-specific PCR primers. From 840 seawater samples, a total of 2 strains of V. harveyi were isolated from shellfish farm seawater. The two isolates were subjected to profiling against 16 antibiotics and found to be resistant to cephalothin, vancomycin, ampicillin, cefepime, cefotetan, and streptomycin. (C) 2014 Elsevier Ltd. All rights reserved.