The effect of electromagnetic field exposure on the formation of DNA lesions
The effect of electromagnetic field exposure on the formation of DNA lesions
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DOI：
10.1179/135100000101535843
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发表时间：
2000-01-01
期刊：
影响因子：
3.8
通讯作者：
Felzenszwalb, I
中科院分区：
文献类型：
--
作者：
da Silva, RL;Albano, F;Felzenszwalb, I
为了确定电磁场(EMF)暴露是否会导致DNA损伤，我们将SnCl2处理的pBR322质粒暴露在EMF中，并使用琼脂糖凝胶电泳法分析了由此产生的构象变化。观察到依赖于电动势的DNA断裂(即松弛质粒的出现)的增强。证实这一点的是，预先暴露在电磁场中的质粒转化大肠杆菌的能力也较弱。结果表明，在过渡金属存在的情况下，电磁场能够引起DNA损伤。这些观察结果支持这样一种观点，即电磁场可能通过第二次产生活性氧物种而产生分裂，并为所观察到的电磁场暴露与人类某些类型癌症的频率之间的相关性提供了可能的解释。
In an attempt to determine whether electromagnetic field (EMF) exposure might lead to DNA damage, we exposed SnCl2-treated pBR322 plasmids to EMF and analysed the resulting conformational changes using agarose gel electrophoresis. An EMF-dependent potentiation of DNA scission (i.e. the appearance of relaxed plasmids) was observed. In confirmation of this, plasmids pre-exposed to EMF also were less capable of transforming Escherichia coli. The results indicate that EMF, in the presence of a transition metal, is capable of causing DNA damage. These observations support the idea that EMF, probably through secondary generation of reactive oxygen species, can be clastogenic and provide a possible explanation for the observed correlation between EMF exposure and the frequency of certain types of cancers in humans.