Evaluation of a method to assess red blood cell aggregation.
Evaluation of a method to assess red blood cell aggregation.
复制标题
评估红细胞聚集方法的评估。
作者：
A. Chabanel;M. Samama
红细胞的可逆聚集在决定血液流动特性中起着重要作用。为了研究影响RBC聚集的不同因素，我们使用了一种新的市售红细胞聚集素（SEFAM，Nancy，France）。该方法允许红细胞聚集动力学的光度定量和聚集体的抗剪切性的估计。红细胞压积的修改主要作用于解聚剪切速率的测定，而血浆组成强烈影响所有测量。抗凝剂本身不影响聚集过程，但可通过血浆稀释效应改变参数值。样本中存在白色血细胞和血小板不会改变数据。对低浓度肝素和低分子量肝素作用的研究表明，在治疗剂量下，这些药物不改变聚集参数值。如果血液样品的处理遵循精确的指导方针，则该方法可用于研究各种疾病中的RBC聚集并寻找适当的血液流变学治疗。
Reversible aggregation of red blood cells (RBC) plays an important role in determining the flow properties of blood. To study different factors affecting RBC aggregation we used a new commercially available erythro-aggregameter (SEFAM, Nancy, France). The method allows the photometric quantitation of the kinetics of RBC aggregation and the estimation of the shear resistance of the aggregates. Modification of the hematocrit acts mostly on the determination of the disaggregation shear rate, while plasma composition strongly affects all measurements. Anticoagulants per se do no influence the aggregation process, but can alter the value of the parameters through a plasma dilution effect. Presence of white blood cells and platelets in the sample did not modify the data. Study on the effects of low concentration of heparin and low molecular weight heparin showed that at therapeutical doses these drugs did not alter the values of the aggregation parameters. Provided that precise guidelines are followed for the processing of blood samples, this method may serve to investigate RBC aggregation in various diseases and to search for adequate hemorheologic treatment.