Capillary electrophoresis with a UV light-emitting diode source for chemical monitoring of native and derivatized fluorescent compounds
Capillary electrophoresis with a UV light-emitting diode source for chemical monitoring of native and derivatized fluorescent compounds
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DOI：
10.1002/elps.200600232
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发表时间：
2006-10-01
期刊：
影响因子：
2.9
通讯作者：
Aspinwall, Craig A.
中科院分区：
文献类型：
--
作者：
Hapuarachchi, Suminda;Janaway, Gordon A.;Aspinwall, Craig A.
我们报告了利用高功率紫外发光二极管荧光检测（UV-LED-IF）在CE分离。CE-UV-LED-IF允许分析一系列环境和生物重要的化合物，包括多环芳烃和生物胺，包括神经递质、氨基酸、蛋白质和肽，这些化合物已经用UV激发的荧光标记物衍生化，例如，邻苯二甲醛/β-巯基乙醇（OPA/β-ME）。365 nm UV-LED用作检测UV激发的荧光化合物的稳定、低成本光源。UV-LED-IF与区带CE分离和MEKC一起使用。多环芳烃的天然荧光检测完成的检测限范围从10 nM到1.3 μ M。OPA/β-ME标记的谷氨酸和天冬氨酸的检测限分别为11和10 nM，分别为离线标记，和47和47 nM，分别为在线标记，相当于紫外激光为基础的系统。对OPA/β-ME标记的蛋白质和肽进行分析，BSA和肌红蛋白的检测限分别为28和47 nM。
We report the utilization of a high power UV light-emifting diode for fluorescence detection (UV-LED-IF) in CE separations. CE-UV-LED-IF allows analysis of a range of environmentally and biologically important compounds, including PAHs and biogenic amines, including neurotransmitters, amino acids, proteins, and peptides, that have been derivatized with UV-excited fluorogenic labels, e.g., o-phthalic dicarboxaldehyde/beta-mercaptoethanol (OPA/beta-ME). The 365 nm UV-LED was used as a stable, low cost source for detection of UV-excited fluorescent compounds. UV-LED-IF was used with both zonal CE separations and MEKC. Native fluorescence detection of PAHs was accomplished with detection limits ranging from 10 nM to 1.3 mu M. Detection limits for OPA/beta-ME-labeled glutamic acid and aspartic acid were 11 and 10 nM, respectively, for off-line labeling, and 47 and 47 nM, respectively, for on-line labeling, comparable to UV-laser-based systems. Analysis of OPA/beta-ME-labeled proteins and peptides was performed with 28 and 47 nM detection limits for BSA and myoglobin, respectively.