Gene-for-gene disease resistance without the hypersensitive response in Arabidopsis dnd1 mutant
Gene-for-gene disease resistance without the hypersensitive response in Arabidopsis dnd1 mutant
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DOI：
10.1073/pnas.95.13.7819
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发表时间：
1998-06-23
影响因子：
11.1
通讯作者：
Bent, AF
中科院分区：
文献类型：
--
作者：
Yu, IC;Parker, J;Bent, AF
细胞死亡反应是植物抗病基因的一个重要特征，被称为过敏反应（HR）。拟南芥的突变株系被鉴定，其中尽管HR细胞死亡的虚拟情况下发生有效的基因对基因抗性。在DND 1基因座突变的植物在HR细胞死亡中是有缺陷的，但保留对无毒假单胞菌的特征性反应，例如诱导病程相关的基因表达和对病原体生长的强烈限制。突变体dnd1植物还表现出对广谱的有毒真菌、细菌和病毒病原体的增强的抗性。在dnd 1植物中对毒性病原体的抗性在数量上不太强，并且可与由抗性基因RPS2和RPM1介导的基因对基因抗性区分。水杨酸化合物和病程相关基因的mRNA水平在dnd1植物中组成性升高。这种系统获得性抗性的组成型诱导可能取代HR细胞死亡，增强更强的基因对基因防御反应。虽然细胞死亡可能有助于野生型植物中的防御信号转导，但dnd 2突变体表明，在拟南芥对P.dingae的基因对基因抗性反应中，在没有广泛的HR细胞死亡的情况下，可以发生对病原体生长的强烈限制。
The cell death response known as the hypersensitive response (HR) is a central feature of gene for gene plant disease resistance. A mutant line of Arabidopsis thaliana was identified in which effective gene-for-gene resistance occurs despite the virtual absence of HR cell death. Plants mutated at the DND1 locus are defective in HR cell death but retain characteristic responses to avirulent Pseudomonas syringae such as induction of pathogenesis-related gene expression and strong restriction of pathogen growth. Mutant dnd1 plants also exhibit enhanced resistance against a broad spectrum of virulent fungal, bacterial, and viral pathogens. The resistance against virulent pathogens in dnd1 plants is quantitatively less strong and is differentiable from the gene-for-gene resistance mediated by resistance genes RPS2 and RPM1. Levels of salicylic acid compounds and mRNAs for pathogenesis-related genes are elevated constitutively in dnd1 plants. This constitutive induction of systemic acquired resistance may substitute for HR cell death in potentiating the stronger gene-for-gene defense response. Although cell death may contribute to defense signal transduction in wild-type plants, the dnd2 mutant demonstrates that strong restriction of pathogen growth can occur in the absence of extensive HR cell death in the gene-for-gene resistance response of Arabidopsis against P. syringae.