Glycation of ovalbumin after high-intensity ultrasound pretreatment: effects on conformation, immunoglobulin (Ig)G/IgE binding ability and antioxidant activity
Glycation of ovalbumin after high-intensity ultrasound pretreatment: effects on conformation, immunoglobulin (Ig)G/IgE binding ability and antioxidant activity
复制标题
高强度超声预处理后卵清蛋白的糖化：对构象、免疫球蛋白 (Ig)G/IgE 结合能力和抗氧化活性的影响
DOI：
10.1002/jsfa.8890
复制
发表时间：
2018-08-15
影响因子：
4.1
通讯作者：
Song, Qidong
中科院分区：
文献类型：
--
作者：
Yang, Wenhua;Tu, Zongcai;Song, Qidong
鸡蛋白蛋白（OVA）是一种具有优良营养和加工性能的蛋白质，是鸡蛋蛋白的主要过敏原。高强度超声治疗通过揭示OVA的构象结构，提高免疫球蛋白（Ig）G和IgE的结合能力。这可能允许通过将高强度超声与其他方法（如糖基化）相结合来修饰卵细胞的IgG和IgE结合，从而代表了改善蛋白质特性的有前途的方法。结果0 ~ 600W超声预处理后糖基化甘露糖(M)可显著降低OVA-M偶联物的IgG和IgE结合能力，显著增强其抗氧化活性，在600W时IgG和IgE结合能力最低，抗氧化能力最高。聚丙烯酰胺凝胶电泳结果显示，超声预处理后OVA-M偶联物的分子量比未预处理样品增加更多，说明超声预处理促进了糖基化。虫卵的-螺旋含量和紫外吸收明显增加，-片含量、本征荧光和表面疏水性明显降低，表明虫卵的三级和二级结构发生了明显变化。结论高强度超声预处理有利于降低IgG和IgE的结合能力，增强OVA-M偶联物的抗氧化活性。因此，糖基化联合高强度超声预处理可能是生产低过敏性、高抗氧化OVA产品的一种有前景的方法。(c) 2018年化学工业学会
BACKGROUNDOvalbumin (OVA), a protein with excellent nutritional and processing properties, is the major allergen of hen egg white. High-intensity ultrasound treatment increases the immunoglobulin (Ig)G and IgE binding abilities by unfolding the conformational structure of OVA. This may allow a modification of the IgG and IgE binding of OVA by combining high-intensity ultrasound with other methods, such as glycation, thus representing a promising method for the improvement of protein properties.RESULTSGlycation with mannose (M) after ultrasound pretreatment at 0-600W significantly reduced the IgG and IgE binding abilities and dramatically enhanced the antioxidant activity of OVA-M conjugates, with the lowest values of IgG and IgE binding and highest values of antioxidant capacity observed at 600W. Polyacrylamide gel electrophoresis showed that the molecular weight of OVA-M conjugates with ultrasound pretreatment increased more than non-pretreatment sample, implying that ultrasound pretreatment promoted glycation. The -helix content and ultraviolet absorption of OVA were observably increased, whereas -sheet content, intrinsic fluorescence and surface hydrophobicity were notably decreased, indicating that the tertiary and secondary structures of OVA were markedly changed.CONCLUSIONHigh-intensity ultrasound pretreatment can be conducive to reducing the binding abilities of IgG and IgE and enhancing the antioxidant activity of OVA-M conjugates. Therefore, glycation combined with high-intensity ultrasound pretreatment might be a promising method for producing hypo-allergenic and high-antioxidant OVA products. (c) 2018 Society of Chemical Industry