Developmental expression of an amn+ transgene rescues the mutant memory defect of amnesiac adults
Developmental expression of an amn+ transgene rescues the mutant memory defect of amnesiac adults
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DOI：
10.1523/jneurosci.19-20-08740.1999
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发表时间：
1999-10-15
影响因子：
5.3
通讯作者：
Tully, T
中科院分区：
文献类型：
--
作者：
DeZazzo, J;Xia, SZ;Tully, T
已经提出果蝇记忆基因amnesiac（amn）编码神经肽蛋白，其包括与脊椎动物垂体腺苷酸环化酶激活肽同源的区域（PACAP; Feany和Quinn，1995）。然而，将这种基因与记忆形成联系起来的连续实验还没有完成（Kandel和Abel，1995）。这里描述的实验表明，假定amn转录参与成人记忆的形成。通过使用UAS-amn（+）转基因，我们显示了amn（28 A）中记忆缺陷的完全拯救，amn（28 A）是由插入GAL 4增强子陷阱转座子引起的突变等位基因（摩尔等人，1998年）。对amn（28 A）报告基因的研究揭示了其在成体脑中的广泛表达，但在胚胎和幼虫神经系统中也有丰富的表达。为了开始解决记忆中amn的时间需求，我们询问是否可以通过将转基因表达限制在成年阶段来挽救记忆缺陷。先前显示的热休克方案完全挽救了amn乙醇敏感性缺陷（摩尔等人，1998）未能挽救记忆缺陷。这些结果，加上先前的遗传和解剖学研究，表明成年记忆形成和乙醇敏感性对amn有不同的时间和空间需求。
The Drosophila memory gene amnesiac (amn) has been proposed to encode a neuropeptide protein, which includes regions homologous to vertebrate pituitary adenylyl cyclase-activating peptide (PACAP; Feany and Quinn, 1995). Definitive experiments to link this gene to memory formation, however, have not yet been accomplished (Kandel and Abel, 1995). The experiments described here demonstrate that the putative amn transcript is involved in adult memory formation. With the use of a UAS-amn(+) transgene, we show complete rescue of memory defects in amn(28A), a mutant allele caused by the insertion of a GAL4 enhancer trap transposon (Moore et al., 1998). Study of the amn(28A) reporter reveals widespread expression in the adult brain but also enriched expression in the embryonic and larval nervous systems. To begin addressing the temporal requirement of amn in memory, we asked whether the memory defects could be rescued by restricting transgenic expression to the adult stage. A heat-shock regimen shown previously to rescue fully the amn ethanol sensitivity defect (Moore et al., 1998) failed to rescue the memory defect. These results, coupled with previous genetic and anatomical studies, suggest that adult memory formation and ethanol sensitivity have different temporal and spatial requirements for amn.