Specific roles for the Ccr4-Not complex subunits in expression of the genome
Specific roles for the Ccr4-Not complex subunits in expression of the genome
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DOI：
10.1261/rna.1348209
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发表时间：
2009-03-01
期刊：
影响因子：
4.5
通讯作者：
Collart, Martine A.
中科院分区：
文献类型：
--
作者：
Azzouz, Nowel;Panasenko, Olesya O.;Collart, Martine A.
在这项工作中，我们使用微阵列实验来确定保守的CCR4-NOT复合体的每个非必要亚基在酿酒酵母基因表达调控中的作用。这项研究是在细胞在高糖中指数生长和细胞生长到葡萄糖耗尽的情况下进行的。当任何给定的亚基缺失时，观察到特定的基因去调控模式，揭示了每个亚基功能的特异性。一致地，通过Caf40p从野生型细胞或缺乏CCR4的单个亚基的细胞中进行串联亲和纯化的CCR4-非复合体的纯化表明，每个亚基对复合体的完整性有特定的影响。此外，微阵列显示，每个亚基的作用是特定于生长条件的。仅从两种不同生长条件的研究揭示了CCR4-非复合体对超过85%的研究基因的影响，我们可以推断CCR4-非复合体对大多数酵母基因组的表达是重要的。
In this work we used micro-array experiments to determine the role of each nonessential subunit of the conserved Ccr4-Not complex in the control of gene expression in the yeast Saccharomyces cerevisiae. The study was performed with cells growing exponentially in high glucose and with cells grown to glucose depletion. Specific patterns of gene deregulation were observed upon deletion of any given subunit, revealing the specificity of each subunit's function. Consistently, the purification of the Ccr4-Not complex through Caf40p by tandem affinity purification from wild-type cells or cells lacking individual subunits of the Ccr4-Not complex revealed that each subunit had a particular impact on complex integrity. Furthermore, the micro-arrays revealed that the role of each subunit was specific to the growth conditions. From the study of only two different growth conditions, revealing an impact of the Ccr4-Not complex on more than 85% of all studied genes, we can infer that the Ccr4-Not complex is important for expression of most of the yeast genome.