Rated-M for mesocosm: allowing the multimodal analysis of mature root systems in 3D.
Rated-M for mesocosm: allowing the multimodal analysis of mature root systems in 3D.
复制标题
DOI：
10.1042/etls20200278
复制
发表时间：
2021-05-21
影响因子：
3.8
通讯作者：
Topp CN
中科院分区：
文献类型：
--
作者：
Dowd T;McInturf S;Li M;Topp CN
植物的水分和养分主要通过根吸收，在自然环境中，这高度依赖于根系的三维构型，统称为根系构型（RSA）。RSA由于各种因素而难以研究，因此，已经开发了一系列方法来解决用于成像的生长根系和成像方法本身的挑战，尽管没有“最佳”方法，因为每个方法都有自己的权衡范围。在这里，我们描述了几种用于植物生长或成像的方法。然后，我们介绍了适应和整合的三个互补的方法，根围生态系统，摄影测量，电阻层析成像（ERT）。中围生态系统可以允许不受约束的根生长，挖掘和保存三维RSA，以及便于使用各种传感器的模块化。恢复的根系可以通过摄影测量进行数字重建，这是一种廉价的方法，只需要一个适当的工作室空间和数码相机。最后，我们演示了如何三维水的可用性可以使用ERT内的根围生态系统进行测量。
A plants’ water and nutrients are primarily absorbed through roots, which in a natural setting is highly dependent on the 3-dimensional configuration of the root system, collectively known as root system architecture (RSA). RSA is difficult to study due to a variety of factors, accordingly, an arsenal of methods have been developed to address the challenges of both growing root systems for imaging, and the imaging methods themselves, although there is no ‘best’ method as each has its own spectrum of trade-offs. Here, we describe several methods for plant growth or imaging. Then, we introduce the adaptation and integration of three complementary methods, root mesocosms, photogrammetry, and electrical resistance tomography (ERT). Mesocosms can allow for unconstrained root growth, excavation and preservation of 3-dimensional RSA, and modularity that facilitates the use of a variety of sensors. The recovered root system can be digitally reconstructed through photogrammetry, which is an inexpensive method requiring only an appropriate studio space and a digital camera. Lastly, we demonstrate how 3-dimensional water availability can be measured using ERT inside of root mesocosms.