Aspects of electrolyte transport across isolated dog retinal pigment epithelium.
Aspects of electrolyte transport across isolated dog retinal pigment epithelium.
复制标题
电解质跨离体狗视网膜色素上皮运输的方面。
DOI：
10.1152/ajprenal.1986.250.5.f781
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
S. Iizuka
中科院分区：
文献类型：
--
作者：
S. Tsuboi;R. Manabe;S. Iizuka
本文研究了Na和Cl在离体狗视网膜色素上皮（RPE）脉络膜中的转运。在短路条件下，观察到从脉络膜到视网膜的净Na通量，并确定在相反方向的净Cl通量。由这两种离子的净通量产生的电流大于短路电流（SCC）。在根尖侧加入10（-5）M哇巴因可抑制Na和Cl的净通量，而使SCC降低84%。在根尖侧加入10（-4）M呋塞米可抑制净Cl通量，但对净Na转运无影响。10（-4）M呋塞米使SCC减少38%。这些药物应用于基底侧时没有效果。因此，Na和Cl的转运依赖于狗RPE顶膜中的Na-K-ATP酶。一个呋塞米敏感的中性载体在顶端的膜建议运输的Cl。用SO 4代替HCO 3在浴溶液中引起SCC的增加，表明HCO 3穿过短路的狗RPE脉络膜的脉络膜到视网膜的移动。
Transport of Na and Cl across the isolated dog retinal pigment epithelium (RPE) choroid was investigated. Under the short-circuit condition, a net Na flux was observed from choroid to retina and a net Cl flux was determined in the opposite direction. The current created by the net flux of these two ions was larger than the short-circuit current (SCC). Addition of 10(-5) M ouabain to the apical side inhibited net fluxes of both Na and Cl, whereas it reduced the SCC 84%. Addition of 10(-4) M furosemide to the apical side inhibited net Cl flux but had no effect on the net Na transport. The 10(-4) M furosemide reduced the SCC 38%. These drugs had no effect when applied to the basal side. Thus the transport of both Na and Cl depends on the Na-K-ATPase in the apical membrane of the dog RPE. A furosemide-sensitive neutral carrier at the apical membrane is suggested for the transport of Cl. Replacement of HCO3 with SO4 in the bathing solution caused an increase in the SCC, indicating the choroid-to-retina movement of HCO3 across the short-circuited dog RPE choroid.