Detection of Tear Components Using Matrix-Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry for Rapid Dry Eye Diagnosis
Detection of Tear Components Using Matrix-Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry for Rapid Dry Eye Diagnosis
复制标题
使用基质辅助激光解吸电离/飞行时间质谱法检测泪液成分以进行快速干眼诊断
DOI：
10.1021/acs.jproteome.0c00138
复制
发表时间：
2020
影响因子：
4.4
通讯作者：
Hao Chen
中科院分区：
文献类型：
--
作者：
Ting Zhang;Liang Hu;Huixiang Ma;Fangfang Ni;Fei Liu;Hao Chen
泪水是一种生物液体，有可能诊断出眼部疾病。细胞外小泡(EVS)广泛存在于包括泪液在内的各种生物体液中，是由活细胞释放的纳米颗粒，被认为是无创液体活检的最有前途的检测源。了解泪液和泪液在干眼等眼科疾病中的作用有助于临床诊断的研究，这通常需要用一种快速有效的方法检测这种液体物体。在这项研究中，我们使用了一种基于质谱学的策略来分析泪液和EV的多肽/蛋白质组图谱，以用于快速干眼诊断。从健康对照组(HC)和干眼综合征(DES)患者的泪液中分离出纳米EV，并利用基质辅助激光解吸电离/飞行时间质谱仪追踪其指纹，进一步分析其泪液成分。可以以剂量依赖的方式观察泪液和泪液的指纹，从而可以比较泪液和泪液之间的鉴别峰。通过对这些峰的分析，泪液和泪液EVS的指纹图谱能够区分DES患者和HC供者，为筛选潜在的DES生物标志物提供了一种有效的方法。所提出的泪液和EV指纹分析方法有望成为快速诊断眼部疾病和深入研究发病机制的一种潜在工具。数据可通过标识符为PXD020217的ProteomeXchange获得。
The tear is a biological fluid that has the diagnostic potential for ocular diseases. Extracellular vesicles (EVs), widly detected in various biofluids including tears, are nanoparticles released by living cells and considered as promising detection sources for noninvasive liquid biopsy. Understanding the roles of tears and tear–EVs in ocular diseases such as dry eye can facilitate the studies of clinical diagnosis, which usually entails detecting such liquid objects with a rapid and effective method. In this study, we used a mass spectrometry–based strategy to analyze peptidome/proteome profiles of tears and EVs for rapid dry eye diagnosis. Nanosized EVs were isolated from tears of both healthy control (HC) individuals and dry eye syndrome (DES) patients, and the tear compositions were further analyzed by tracking their fingerprints with matrix-assisted laser desorption ionization/time-of-flight mass spectrometry. The fingerprints of tear–EVs could be observed in a dose-dependent manner and tears, allowing for comparison of the discriminant peaks between tears and EVs. By analyzing these peaks, the fingerprints of both tear and tear–EVs were showed to have the capability of distinguishing patients with DES from HC donors and providing an efficient way for screening potential DES biomarkers. The proposed tear and EV fingerprinting approach is expected to be a potential tool in the rapid diagnosis of ocular diseases and in-depth research on pathogenesis. Data are available via ProteomeXchange with identifier PXD020217.