IMMUNOCHEMICAL CHARACTERIZATION OF RECOMBINANT AND NATIVE TROPOMYOSINS AS A NEW ALLERGEN FROM THE HOUSE-DUST MITE, DERMATOPHAGOIDES-FARINAE
IMMUNOCHEMICAL CHARACTERIZATION OF RECOMBINANT AND NATIVE TROPOMYOSINS AS A NEW ALLERGEN FROM THE HOUSE-DUST MITE, DERMATOPHAGOIDES-FARINAE
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DOI：
10.1016/s0091-6749(95)70035-8
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发表时间：
1995-07-01
影响因子：
14.2
通讯作者：
ONO, K
中科院分区：
文献类型：
--
作者：
AKI, T;KODAMA, T;ONO, K
背景资料：两种主要螨种，粉尘螨和屋尘螨，负责速发型超敏反应。虽然大量的抗原和过敏原中检测到整个螨培养提取物，只有主要的过敏原的Der I和II组已被广泛研究。方法：从一株D.花粉蛋白cDNA文库原肌球蛋白基因的表达产物与D.结果：从该cDNA克隆中推导出的氨基酸序列与多种动物中保守的原肌球蛋白具有很高的同源性。纯化蛋白经溴化氰降解得到的两个片段的氨基酸序列与推导的氨基酸序列的相应部分完全一致。原肌球蛋白与特异性IgE的反应率为80.6%，与Der f Ⅰ（90.3%）和Der f Ⅱ（74.2%）相当。特异性IgE抗体对重组和天然原肌球蛋白的高结合反应表明，螨原肌球蛋白是屋尘螨中另一种重要的过敏原。
Background: Two predominant mite species, Dermatophagoides farinae and Dermatophagoides pteronyssinus, are responsible for the immediate hypersensitivity reaction. Although a large number of antigens and allergens was detected in a whole mite culture extract, only the major allergens of Der I and II groups have been extensively studied. Much information on other important allergens remains to be accumulated.Methods: A new allergen complementary DNA (cDNA) clone was isolated from a D. farinae protein cDNA library. The expression product of the cDNA and native tropomyosin from D. farinae were purified, characterized, and evaluated.Results: An amino acid sequence deduced from the cDNA clone revealed significant homology with tropomyosins conserved in a wide range of animals. The amino acid sequences of two fragments obtained by degradation of the purified protein with cyanogen bromide coincided completely with the corresponding portions of the deduced amino acid sequence. The native tropomyosin reacted with specific IgE in the 31 sera tested at a high frequency (80.6%), comparable to that of Der f I (90.3%) and Der f II (74.2%).Conclusions: The cloned cDNA encodes tropomyosin. The high binding response of specific IgE antibodies to recombinant and native tropomyosins demonstrates that mite tropomyosin is an additional important allergen in house dust mite.