Identification and characterization of two contiguous operons required for aerobactin transport and biosynthesis in Vibrio mimicus
Identification and characterization of two contiguous operons required for aerobactin transport and biosynthesis in Vibrio mimicus
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DOI：
10.1111/j.1348-0421.2004.tb03528.x
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发表时间：
2004-01-01
影响因子：
2.6
通讯作者：
Yamamoto, S
中科院分区：
文献类型：
--
作者：
Moon, YH;Tanabe, T;Yamamoto, S
作为对铁剥夺的反应，拟态弧菌产生作为主要铁载体的需氧肌动蛋白。应用毛皮滴定法测定拟态弧菌基因组DNA文库，然后进一步克隆周围区域，导致两个相邻的铁调节操纵子的鉴定。其中一个含有三个编码大肠杆菌FhuCDB同源物的基因，另一个含有五个编码大肠杆菌FhuCDB同源物的基因。coli IucABCD IutA.在各自的操纵子中构建V mimicus极性破坏剂使我们能够确认它们的功能。嗜氧菌介导的铁收购系统在V mimicus的遗传安排是独特的，在嗜氧菌素操纵子（iucABCD iutA）是连续的操纵子（matCDB）编码的ATP结合盒运输系统的铁嗜氧菌素的组件。这是第一份报告表明，需氧肌动蛋白的运输和生物合成基因存在于一个家庭以外的肠杆菌科物种。
In response to iron deprivation, Vibrio mimicus produces aerobactin as a major siderophore. Application of the Fur titration assay to a V mimicus genomic DNA library followed by further cloning of the surrounding regions led to the identification of two adjacent, iron-regulated operons. One contains three genes encoding homologs of the Escherichia coli FhuCDB and the other, five genes encoding homologs of the E. coli IucABCD IutA. Construction of the V mimicus polar disruptants in the respective operons allowed us to confirm their functions. The genetic arrangement of the aerobactin-mediated iron acquisition system in V mimicus is unique in that the aerobactin operon (iucABCD iutA) is contiguous to the operon (matCDB) encoding components of an ATP-binding cassette transport system for ferric aerobactin. This is the first report demonstrating that aerobactin transport and biosynthesis genes are present in a species outside the family Enterobacteriaceae.