MORPHOLOGY AND DEVELOPMENT OF RIFT-VALLEY FEVER VIRUS IN VERO CELL-CULTURES
MORPHOLOGY AND DEVELOPMENT OF RIFT-VALLEY FEVER VIRUS IN VERO CELL-CULTURES
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DOI：
10.1002/jmv.1890240205
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发表时间：
1988-02-01
影响因子：
12.7
通讯作者：
SIMPSON, DIH
中科院分区：
文献类型：
--
作者：
ELLIS, DS;SHIRODARIA, PV;SIMPSON, DIH
裂谷热病毒（RVFV）在Vero细胞培养物中生长，在13小时内完成复制周期。最初的迹象是出现核内纤维杆，随后是宿主细胞质中的前体病毒物质的聚集和病毒核衣壳出芽进入与高尔基体相关的空泡。成熟的颗粒，释放的Vero细胞解体，包含核糖体状结构的核衣壳内，这是由一个典型的单位膜，通过插入约350-375个表面尖峰，其内端被纳入核衣壳结构的包围。在阴性染色的材料中，病毒粒子的总直径为90-110 nm;刺突长度为10-18 nm，直径为5 nm。
Rift Valley fever virus (RVFV) grown in Vero cell cultures has a completed replication cycle within 13 hours. The first signs are the appearance of intranuclear fibrillar rods, followed by aggregations of precursor viral material in host cell cytoplasm and viral nucleocapsids budding into vacuoles associated with the Golgi apparati. Mature particles, liberated by the disintegration of Vero cells, contained ribosomelike structures within the nucleocapsid, which was surrounded by a typical unit membrane through which were inserted some 350-375 surface spikes whose inner ends were incorporated into the nucleocapsid structure. In the negatively stained material, the overall diameter of the virion was 90-110 nm; the spikes were 10-18 nm in length and 5 nm in diameter.