Whole-body cortisol is an indicator of crowding stress in adult zebrafish, Danio rerio
Whole-body cortisol is an indicator of crowding stress in adult zebrafish, Danio rerio
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DOI：
10.1016/j.aquaculture.2006.04.020
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发表时间：
2006-08-31
期刊：
影响因子：
4.5
通讯作者：
Schreck, Carl B.
中科院分区：
文献类型：
--
作者：
Ramsay, Jennifer M.;Feist, Grant W.;Schreck, Carl B.
血浆皮质醇水平已被用来评估各种养殖鱼类的应激反应。然而，人们对斑马鱼的应激反应知之甚少，尽管它被广泛用作实验室研究生物。由于其体积小，全身皮质醇的评估提供了一种手段来评估斑马鱼的压力水平了解拥挤对斑马鱼全身皮质醇的作用将使研究人员能够优化各种饲养程序。本研究的目的是确定斑马鱼全身皮质醇对拥挤应激的反应。尚未确定斑马鱼的基线皮质醇值;因此，我们比较了适应类似条件的特定处理组。我们拥挤的斑马鱼在高密度（40鱼/L）3小时（急性应激），或5天（慢性应激）。拥挤导致平均全身皮质醇水平的四倍增加，在这两个群体相比，斑马鱼保持在一个低得多的密度（0.25鱼/升）。另外的实验表明，皮质醇对拥挤的反应是由禁食，喂养和密度调制。在大玻璃水族箱（76 L），禁食，拥挤的鱼（40鱼/L）有显着较高的皮质醇相比，禁食，控制鱼（0.2鱼/L）。此外，体重与皮质醇水平呈负相关，在禁食，拥挤的鱼在大玻璃水族馆举行。对于喂鱼，拥挤并没有显着增加皮质醇水平，这表明喂食和拥挤之间的相互作用。在小水箱（4 L），拥挤（40鱼/L）没有显着增加皮质醇相比，控制鱼保持在4鱼/L。我们的研究结果表明，全身皮质醇是一个有用的指标拥挤压力禁食，成年斑马鱼。了解拥挤和其他环境条件如何影响斑马鱼的健康，可以帮助优化斑马鱼的生长，健康和繁殖，以及提高使用这种流行的脊椎动物模型的体内研究的一致性和可重复性。(c)2006 Elsevier B. V.保留所有权利。
Plasma cortisol levels have been used to evaluate the stress response in a variety of cultured fish species. However, little is known about the stress response of zebrafish, Danio rerio, despite its extensive use as a laboratory research organism. Due to its small size, evaluation of whole-body cortisol has provided a means to assess zebrafish stress levels Understanding the role of crowding on zebrafish whole-body cortisol would allow researchers to optimize various rearing procedures. The aim of this study was to determine the zebrafish whole-body cortisol response to crowding stress. Baseline cortisol values have not been established for zebrafish; therefore, we compared specific treatment groups acclimated to similar conditions. We crowded zebrafish at a high density (40 fish/L) for 3 h (acute stress), or 5 days (chronic stress). Crowding resulted in a four-fold increase in mean whole-body cortisol level in both groups compared to zebrafish maintained at a much lower density (0.25 fish/L). Additional experiments demonstrated that the cortisol response to crowding was modulated by fasting, feeding and density. In large glass aquaria (76 L), fasted, crowded fish (40 fish/L) had significantly higher cortisol compared to fasted, control fish (0.2 fish/L). Furthermore, weight was inversely related to cortisol level in fasted, crowded fish held in large glass aquaria. For fed fish, crowding did not significantly increase cortisol level, suggesting an interaction between feeding and crowding. In small tanks (4 L), crowding (40 fish/L) did not significantly increase cortisol compared to control fish held at 4 fish/L. Our results suggest that whole-body cortisol is a useful indicator of crowding stress in fasted, adult zebrafish. Understanding how crowding and other environmental conditions affect zebrafish fitness could aid in optimizing zebrafish growth, health and reproduction as well as improving the consistency and reproducibility of in vivo studies that use this popular vertebrate model. (c) 2006 Elsevier B.V. All rights reserved.