Optical method for quantifying rates of mucus secretion from single submucosal glands
Optical method for quantifying rates of mucus secretion from single submucosal glands
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DOI：
10.1152/ajplung.2001.281.2.l458
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发表时间：
2001-08-01
影响因子：
4.9
通讯作者：
Wine, JJ
中科院分区：
文献类型：
--
作者：
Joo, NS;Wu, JV;Wine, JJ
我们描述了一种定量单腺体分泌物的光学方法。将分离的气管粘膜固定在空气-Krebs界面上，涂油。腺体分泌物形成球形气泡，每隔一段时间进行数字成像，从而计算出分泌物的速度。我们对12只绵羊的54个实验中的340个腺体进行了监测。腺体在采后9h内基本以低速率分泌(0.57+/-0.04nL.min(-1)，腺体(-1)，123个腺体)，最长持续3天。卡巴胆碱(10微米)刺激分泌有一个早期的瞬时和一个持续或振荡的阶段。峰值分泌量为15.7+/-1.2nl.min(-1)，腺体(-1)为60个腺体，持续分泌量为4.5+/-0.5nl.min(-1)，腺体(-1)(10个腺体)。异丙肾上腺素和苯肾上腺素(各10 mM)只能刺激小而短暂的反应。我们证实猫对苯肾上腺素有很大的分泌反应(11.6+/-3.7nL.min(-1).gland(-1)，12个腺体)，但猪、羊和人类都有小的反应(
We describe an optical method to quantify single-gland secretion. Isolated tracheal mucosa were mounted at the air-Krebs interface and coated with oil. Gland secretions formed spherical bubbles that were digitally imaged at intervals, allowing rates of secretion to be calculated. We monitored 340 glands in 54 experiments with 12 sheep. Glands secreted basally at low rates (0.57 +/- 0.04 nl.min(-1).gland(-1), 123 glands) in tissues up to 9 h postharvest and at lower rates for up to 3 days. Carbachol (10 muM) stimulated secretion with an early transient and a sustained or oscillating phase. Peak secretion was 15.7 +/- 1.2 nl.min(-1).gland(-1) (60 glands); sustained secretion was 4.5 +/- 0.5 nl.min(-1).gland(-1) (10 glands). Isoproterenol and phenylephrine (10 mM each) stimulated only small, transient responses. We confirmed that cats have a large secretory response to phenylephrine (11.6 +/- 3.7 nl.min(-1).gland(-1), 12glands),but pigs, sheep, and humans all have small responses (