Changes of total water and sucrose space accompanying induced ion uptake or phosphate swelling of rat liver mitochondria.
Changes of total water and sucrose space accompanying induced ion uptake or phosphate swelling of rat liver mitochondria.
复制标题
总水和蔗糖空间的变化伴随着大鼠肝线粒体的诱导离子摄取或磷酸盐肿胀。
作者：
E. J. Harris;K. van Dam
1.测定大鼠肝线粒体在缬氨霉素诱导K(+)摄取和尼日利亚霉素释放K(+)过程中可与氚化水交换的总水量和蔗糖空间。 K(+)含量和蔗糖不可及水一起升降。 2. 在高 K(+) 浓度的介质中添加磷酸盐引起的膨胀主要与蔗糖可及水的增加有关，其中携带溶解的 K(+)。通过添加 ATP 可以逆转这种变化。 3. 如果假定线粒体 K(+) 与一价阴离子一起存在于该空间中，则蔗糖无法进入的空间对渗透压变化的响应在质量上是预期的。 4. 结果表明，光散射法无法区分蔗糖空间和蔗糖不可及空间的变化，而在本实验中，磷酸盐（在高K(+)溶液中）和抗生素诱导的阳离子摄取可分别改变蔗糖空间和蔗糖不可及空间的变化。
1. Total water exchangeable with tritiated water and sucrose space were measured in rat liver mitochondria during the uptake of K(+) induced by valinomycin and the release caused by nigericin. The K(+) content and the sucrose-inaccessible water rose and fell together. 2. Swelling resulting from phosphate addition in a medium of high K(+) concentration was associated mainly with increased sucrose-accessible water, which carried dissolved K(+). This change was reversed by addition of ATP. 3. The response of the sucrose-inaccessible space to changed osmolarity was qualitatively that expected if the mitochondrial K(+) is assumed to be present in this space with a univalent anion. 4. It is brought out that the light-scattering method fails to distinguish between changes in sucrose space and in sucrose-inaccessible space, which in the present experiments could be altered respectively by phosphate (in high K(+) solution) and by cation uptake induced by antibiotic.