Achieving functional neuronal dendrite structure through sequential stochastic growth and retraction.
Achieving functional neuronal dendrite structure through sequential stochastic growth and retraction.
复制标题
DOI：
10.7554/elife.60920
复制
发表时间：
2020-11-26
期刊：
影响因子：
7.7
通讯作者：
Cuntz H
中科院分区：
文献类型：
--
作者：
Ferreira Castro A;Baltruschat L;Stürner T;Bahrami A;Jedlicka P;Tavosanis G;Cuntz H
第I类腹侧后树突状分支(C1vpda)本体感觉神经元在爬行过程中对果蝇幼虫体壁的收缩做出反应。它们的树突分支沿着收缩的方向运行，这可能是爬行收缩过程中最大化膜曲率的功能要求。尽管c1vpda中树枝状图案的分子机制已经被广泛研究，但导致其梳状形状的精确阐述的过程仍然难以捉摸。在这里，为了将树突形状与其本体感觉作用联系起来，我们对c1vpda胚胎和幼虫的形态发生进行了长期的、非侵入性的在体时间推移成像，以揭示一系列的分化阶段。我们将计算机模型和树枝动态跟踪相结合，提出了随机生长和收缩的不同顺序阶段在导线和功能方面都实现了有效的树状结构。我们的研究展示了树枝晶生长如何平衡结构-功能要求，为功能专门化树枝晶自组织的一般原理提供了新的线索。
Class I ventral posterior dendritic arborisation (c1vpda) proprioceptive sensory neurons respond to contractions in the Drosophila larval body wall during crawling. Their dendritic branches run along the direction of contraction, possibly a functional requirement to maximise membrane curvature during crawling contractions. Although the molecular machinery of dendritic patterning in c1vpda has been extensively studied, the process leading to the precise elaboration of their comb-like shapes remains elusive. Here, to link dendrite shape with its proprioceptive role, we performed long-term, non-invasive, in vivo time-lapse imaging of c1vpda embryonic and larval morphogenesis to reveal a sequence of differentiation stages. We combined computer models and dendritic branch dynamics tracking to propose that distinct sequential phases of stochastic growth and retraction achieve efficient dendritic trees both in terms of wire and function. Our study shows how dendrite growth balances structure–function requirements, shedding new light on general principles of self-organisation in functionally specialised dendrites.