Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy
Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy
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DOI：
10.1007/978-1-0716-0621-6_16
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发表时间：
2020-01-01
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影响因子：
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通讯作者：
Allison, Gordon G.
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文献类型：
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作者：
da Costa, Ricardo M. F.;Barrett, William;Allison, Gordon G.
衰减全反射傅里叶变换中红外光谱法（ATR-FTIR）广泛应用于生物材料的化学分析，相对便宜，只需要简单的样品制备，与传统的湿化学或色谱方法相比具有较高的通量。它特别适合于干燥和精细研磨的植物样品的无损分析，用于随后使用化学计量回归模型预测细胞壁和其他成分或加工参数。此外，通过非回归方法（如主成分分析）对中红外光谱进行分析，为实验、加工和环境处理的影响以及基因型和时间差异对化学成分（包括细胞壁成分的变化）的影响提供了一种直接的多变量比较方法。因此，ATR-FTIR在木质纤维素生物质工业的多个层面上具有巨大的潜力。在这里，我们描述了细胞壁样品的制备和ATR-FTIR光谱的生成方法，并提出了在分析样品之间进行统计分析和/或化学计量学模式识别的技术。
Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods. It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models. Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition. There is thus great potential for using ATR-FTIR in the lignocellulosic biomass industry at a number of levels. Here we describe methods for cell wall sample preparation and generation of ATR-FTIR spectra, and suggest techniques for the statistical analysis and/or chemometric pattern recognition between the analyzed samples.