Plasma-deposited tetraglyme surfaces greatly reduce total blood protein adsorption, contact activation, platelet adhesion, platelet procoagulant activity, and in vitro thrombus deposition
Plasma-deposited tetraglyme surfaces greatly reduce total blood protein adsorption, contact activation, platelet adhesion, platelet procoagulant activity, and in vitro thrombus deposition
复制标题
DOI：
10.1002/jbm.a.31091
复制
发表时间：
2007-06-15
影响因子：
4.9
通讯作者：
Horbett, Thomas A.
中科院分区：
文献类型：
--
作者：
Cao, Lan;Chang, Mark;Horbett, Thomas A.
采用纤维蛋白原和血管性血血病因子吸附、总蛋白吸附、再钙化血浆凝血时间、血小板粘附和促凝剂活性、紊乱血流导管模型全血血栓形成等六种体外方法，研究了具有超低纤维蛋白原吸附的四聚乙二醇二甲醚（tetrlyme）血浆沉积涂层降低血液活化的能力。表面等离子体共振结果表明，四肽表面强烈抵抗人体血浆中所有蛋白质的吸附。与对照组相比，存在四甲酰胺表面的凝血时间延长，表明内在凝血级联的激活较低。与未包被和Biospan (R)包被的载玻片相比，四烯酰胺包被材料上的血小板粘附和凝血酶生成大大减少。在体外血流紊乱模型中，四肽血浆包被导管的血栓比未包被导管少50%。因此，通过所有六种方法测量，四烯酰胺涂层材料大大减少了血液相互作用。因此，血浆沉积的四甲酰胺改善了血液相容性，不仅是因为它们减少了血小板粘附和活化，而且还因为血液相互作用的普遍减少。(c) 2007 Wiley期刊公司[J] .中国生物医学工程学报，2016,31(1):557 - 557。
The ability of tetraethylene glycol dimethyl ether (tetraglyme) plasma deposited coatings exhibiting ultralow fibrinogen adsorption to reduce blood activation was studied with six in vitro methods, namely fibrinogen and von Willebrand's factor adsorption, total protein adsorption, clotting time in recalcified plasma, platelet adhesion and procoagulant activity, and whole blood thrombosis in a disturbed flow catheter model. Surface plasmon resonance results showed that tetraglyme surfaces strongly resisted the adsorption of all proteins from human plasma. The clotting time in the presence of tetraglyme surfaces was lengthened compared with controls, indicating a lower activation of the intrinsic coagulation cascade. Platelet adhesion and thrombin generation by adherent platelets were greatly reduced on tetraglyme-coated materials, compared with uncoated and Biospan (R)-coated glass slides. in the in vitro disturbed blood flow model, tetraglyme plasma coated catheters had 50% less thrombus than did the uncoated catheters. Tetraglyme-coated materials thus had greatly reduced blood interactions as measured with all six methods. The improved blood compatibility of plasma-deposited tetraglyme is thus not only due to their reduced platelet adhesion and activation, but also to a generalized reduction in blood interactions. (c) 2007 Wiley Periodicals, Inc. J Biomed Mater Res 81A: 827-837, 2007.