Functional Recovery after Scutellarin Treatment in Transient Cerebral Ischemic Rats: A Pilot Study with (18) F-Fluorodeoxyglucose MicroPET.
Functional Recovery after Scutellarin Treatment in Transient Cerebral Ischemic Rats: A Pilot Study with (18) F-Fluorodeoxyglucose MicroPET.
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DOI：
10.1155/2013/507091
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Li JH;Lu J;Zhang H
Objective.采用18氟脱氧葡萄糖（18 F-FDG）微正电子发射断层扫描（microPET）技术研究灯盏乙素（Scu）对脑缺血大鼠的神经保护作用。法采用大脑中动脉闭塞法建立脑缺血模型。将大鼠随机分为5组：假手术组、脑缺血再灌注未处理组（CIRU组）、Scu-25组（Scu 25 mg/kg/d）、Scu-50组（Scu 50 mg/kg/d）和尼莫地平组（Nimodipine 10 mg/Kg/d）。两组均连续用药2周。以脑梗死体积、神经功能缺损评分和脑葡萄糖代谢为指标评价治疗效果。采用免疫组化法检测血管密度因子（vWF）、胶质细胞标志物（GFAP）和成熟神经元标志物（NeuN）的水平。结果Scu-50组神经功能缺损评分较CIRU组显著降低（P < 0.001）。Scu-50组与CIRU组（P < 0.01）和Scu-25组（P <0.01）比较，18F-FDG在梗死侧的蓄积量随时间逐渐增加。免疫组化分析表明Scu-50促进神经元成熟。结论18F-FDG microPET成像显示，在脑缺血大鼠模型中，Scu-50治疗后代谢恢复。Scu对脑缺血损伤的神经保护作用可能与其增加局部葡萄糖活性和促进神经元成熟有关。
Objective. To investigate neuroprotective effects of scutellarin (Scu) in a rat model of cerebral ischemia with use of 18F-fluorodeoxyglucose (18F-FDG) micro positron emission tomography (microPET). Method. Middle cerebral artery occlusion was used to establish cerebral ischemia. Rats were divided into 5 groups: sham operation, cerebral ischemia-reperfusion untreated (CIRU) group, Scu-25 group (Scu 25 mg/kg/d), Scu-50 group (Scu 50 mg/kg/d), and nimodipine (10 mg/Kg/d). The treatment groups were given for 2 weeks. The therapeutic effects in terms of cerebral infarct volume, neurological deficit scores, and cerebral glucose metabolism were evaluated. Levels of vascular density factor (vWF), glial marker (GFAP), and mature neuronal marker (NeuN) were assessed by immunohistochemistry. Results. The neurological deficit scores were significantly decreased in the Scu-50 group compared to the CIRU group (P < 0.001). 18F-FDG accumulation in the ipsilateral cerebral infarction increased steadily over time in Scu-50 group compared with CIRU group (P < 0.01) and Scu-25 group (P < 0.01). Immunohistochemical analysis demonstrated Scu-50 enhanced neuronal maturation. Conclusion. 18F-FDG microPET imaging demonstrated metabolic recovery after Scu-50 treatment in the rat model of cerebral ischemia. The neuroprotective effects of Scu on cerebral ischemic injury might be associated with increased regional glucose activity and neuronal maturation.
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DOI：
10.1038/jcbfm.2010.97
发表时间：
2011-01
期刊：
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
影响因子：
--
作者：
通讯作者：
--
影响因子：
8.2
作者：
Guo, Li-li;Guan, Zhi-zhong;Wang, Yong-lin
通讯作者：
Wang, Yong-lin
影响因子：
8.3
作者：
Minnerup, Jens;Kim, Jeong Beom;Schaebitz, Wolf-Ruediger
通讯作者：
Schaebitz, Wolf-Ruediger
影响因子：
8.3
作者：
Gürsoy-Özdemir, Y;Can, A;Dalkara, T
通讯作者：
Dalkara, T
影响因子：
6.3
作者：
Hayakawa, Kazuhide;Nakano, Takafumi;Fujiwara, Michihiro
通讯作者：
Fujiwara, Michihiro