Effects of hypoxia on energy state and pH in resting pulmonary and femoral arterial smooth muscles.
Effects of hypoxia on energy state and pH in resting pulmonary and femoral arterial smooth muscles.
复制标题
缺氧对静息肺动脉和股动脉平滑肌能量状态和 pH 值的影响。
DOI：
10.1152/ajplung.1998.275.6.l1051
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Sylvester,JT
中科院分区：
文献类型：
--
作者：
Leach,RM;Sheehan,DW;Chacko,VP;Sylvester,JT
To determine the effects of hypoxia on energy state and intracellular pH (pHi) in resting pulmonary and systemic arterial smooth muscles, we used31P nuclear magnetic resonance spectroscopy and colorimetric and enzymatic assays to measure pHi; intracellular concentrations of ATP, phosphocreatine, creatine, and Pi; and phosphorylation potential in superfused tissue segments from porcine proximal intrapulmonary and superficial femoral arteries. Under baseline conditions (467 ± 12.1 mmHg), energy state and total creatine (phosphocreatine + creatine) concentration were lower and pHiwas higher in pulmonary arteries. During hypoxia (23 ± 2.4 mmHg), energy state deteriorated more in femoral arteries than in pulmonary arteries. pHifell in both tissues but was always more alkaline in pulmonary arteries. Reoxygenation reversed the changes induced by hypoxia. These results suggest that production and/or elimination of ATP and H+was different in resting pulmonary and systemic arterial smooth muscles under baseline and hypoxic conditions. Because energy state and pHiaffect a wide variety of cellular processes, including signal transduction, contractile protein interaction, and activities of ion pumps and channels, further investigation is indicated to determine whether these differences have functional significance.
