Preliminary measurements of optical properties in human abscess cavities prior to methylene blue photodynamic therapy.
Preliminary measurements of optical properties in human abscess cavities prior to methylene blue photodynamic therapy.
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亚甲蓝光动力治疗前人体脓肿腔光学特性的初步测量。
DOI：
10.1117/12.2648453
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Baran,TimothyM
中科院分区：
文献类型：
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作者：
Hannan,MdNafiz;Sharma,AshwaniK;Baran,TimothyM
作为我们正在进行的1期临床试验的一部分，以确定亚甲蓝光动力疗法（MB-PDT）对人类深部组织脓肿腔的安全性和可行性，我们已经表明，脓肿壁光学特性的确定对于旨在优化光剂量的个性化治疗计划的设计至关重要。为此，我们开发并验证了一种用于评估腔壁光学特性的光谱系统，包括一种紧凑型光纤探头，可通过用于标准护理脓肿引流的导管插入。在这里，我们报告了前三个人类受试者接受这些光谱测量的初步结果。我们观察到MB给药前氧合血红蛋白和脱氧血红蛋白浓度的广泛变异性，范围分别为7.3-213 μM和0.1-47.2 μM。降低散射系数也显示患者间变异性，但受试者之间的恢复值更相似（665 nm处为5.5-10.9 cm-1）。此外，发现受试者之间的亚甲蓝摄取不同，并且与氧饱和度的降低相关。这些测量的光学特性，沿着术前计算机断层扫描（CT）图像，将与我们先前开发的Monte Carlo模拟框架一起用于为个体患者生成个性化治疗计划，这可以显着提高MB-PDT的疗效，同时确保安全性。
As part of our ongoing Phase 1 clinical trial to establish the safety and feasibility of methylene blue photodynamic therapy (MB-PDT) for human deep tissue abscess cavities, we have shown that determination of abscess wall optical properties is vital for the design of personalized treatment plans aiming to optimize light dose. To that end, we have developed and validated an optical spectroscopy system for the assessment of optical properties at the cavity wall, including a compact fiber-optic probe that can be inserted through the catheter used for the standard of care abscess drainage. Here we report preliminary findings from the first three human subjects to receive these optical spectroscopy measurements. We observed wide variability in concentrations of oxy- and deoxy-hemoglobin prior to MB administration, ranging from 7.3-213 μM and 0.1-47.2 μM, respectively. Reduced scattering coefficients also showed inter-patient variability, but recovered values were more similar between subjects (5.5-10.9 cm-1at 665 nm). Further, methylene blue uptake was found to vary between subjects, and was associated with a reduction in oxygen saturation. These measured optical properties, along with preprocedure computed tomography (CT) images, will be used with our previously developed Monte Carlo simulation framework to generate personalized treatment plans for individual patients, which could significantly improve the efficacy of MB-PDT while ensuring safety.