Effect of basic fibroblast growth factor on left ventricular geometry in rats subjected to coronary occlusion and reperfusion.
Effect of basic fibroblast growth factor on left ventricular geometry in rats subjected to coronary occlusion and reperfusion.
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发表时间：
2002-02
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通讯作者：
M. Scheinowitz;A. Kotlyar;S. Zimand;I. Leibovitz;N. Varda‐Bloom;D. Ohad;I. Goldberg;S. Engelberg;N. Savion;M. Eldar
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作者：
M. Scheinowitz;A. Kotlyar;S. Zimand;I. Leibovitz;N. Varda‐Bloom;D. Ohad;I. Goldberg;S. Engelberg;N. Savion;M. Eldar
背景技术先前的研究已证明在慢性心肌缺血或急性心肌梗塞后施用碱性成纤维细胞生长因子可挽救心肌。目的 研究 bFGF 对大鼠冠状动脉闭塞和再灌注后左心室形态测量的影响。方法 使用植入式渗透泵连续施用 bFGF（0.5 mg）或安慰剂一周。手术后6周处死动物，并用Massontrichrome和抗增殖细胞核抗原抗体对心肌横截面进行染色。结果 与对照组动物相比，左室面积、左室腔直径、左室腔/壁厚度比和损伤大小没有变化。与正常心肌相比，受损心肌中增殖的内皮细胞明显更丰富，但用bFGF治疗或未治疗的动物之间没有差异。结论 冠状动脉闭塞和再灌注后全身性 bFGF 给药一周对大鼠左心室几何形状或细胞增殖没有额外影响。
BACKGROUND Previous studies have demonstrated myocardial salvage by basic fibroblast growth factor administration following chronic myocardial ischemia or acute myocardial infarction. OBJECTIVES To study the effect of bFGF on left ventricular morphometry following coronary occlusion and reperfusion episode in rats. METHODS bFGF (0.5 mg) or placebo was continuously administered for a period of one week using an implanted osmotic pump. Animals were sacrificed 6 weeks after surgery and myocardial cross-sections were stained with Massontrichrome and with anti-proliferating cell nuclear antigen antibody. RESULTS LV area, LV cavity diameter, LV cavity/wall thickness ratio, and injury size were unchanged compared with control animals. Proliferating endothelial cells were significantly more abundant in injured compared with normal myocardium, but with no differences between animals treated or not treated with bFGF. CONCLUSIONS One week of systemic bFGF administration following coronary occlusion and reperfusion had no additional effect on LV geometry or cellular proliferation in rats.