The regulation of high insulin levels on ovary apoptosis in early pregnant mice.
The regulation of high insulin levels on ovary apoptosis in early pregnant mice.
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DOI：
10.1016/j.bbrc.2016.12.041
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发表时间：
2017-01
影响因子：
3.1
通讯作者：
Chen Zhang;Juan Wu;Junlin He;Xue-qing Liu;Xuemei Chen;C. Tong;Yubin Ding;Yanqing Geng;Wenqi Chen;Yingxiong Wang;Rufei Gao
中科院分区：
文献类型：
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作者：
Chen Zhang;Juan Wu;Junlin He;Xue-qing Liu;Xuemei Chen;C. Tong;Yubin Ding;Yanqing Geng;Wenqi Chen;Yingxiong Wang;Rufei Gao
研究表明，代谢综合征与生殖问题有关。患有以高胰岛素血症为特征的代谢综合征的女性常见卵巢功能障碍，但其机制仍不清楚。本研究的目的是探讨妊娠早期高水平胰岛素对卵巢功能的影响。高胰岛素暴露组的小鼠皮下注射人重组胰岛素。胰岛素治疗后，使用ELISA试剂盒测试了各种激素的变化，结果显示胰岛素组卵巢分泌的激素发生了显着变化。 TUNEL染色显示胰岛素组TUNEL阳性细胞较少。免疫组织化学研究发现，胰岛素组卵巢中 Bax 显着减少，Bcl2 增加。 Western blotting结果显示胰岛素组卵巢中凋亡相关蛋白表达明显改变。此外，胰岛素组卵巢中 p-Akt 蛋白的表达显着上调。此外，Akt抑制剂LY294002逆转了早孕小鼠卵巢组织中高胰岛素的抗凋亡作用。所有这些结果都表明妊娠早期胰岛素损害卵巢功能，卵巢细胞凋亡在胰岛素作用下失衡。 PI3K/AKT信号通路可能参与这一过程。
Studies have shown that metabolic syndrome is associated with reproductive problems. Women with metabolic syndrome, characterized by hyperinsulinemia, have common ovarian dysfunction, but the mechanism remains elusive. The aim of this research is to explore the effects of high levels of insulin on ovary function during early pregnancy. Mice in the high insulin-exposed group were given a subcutaneous injection of human recombinant insulin. After insulin treatment, changes in various hormones were tested using ELISA kits which showed hormones secreted by the ovary were significantly altered in the insulin group. TUNEL staining showed less TUNEL-positive cells in the insulin group. A significant decrease in Bax and an increase in Bcl2 in the ovary were found in the insulin group by immunohistochemical studies. Western blotting showed the expressions of apoptosis related proteins in the ovaries from the insulin group were obviously altered. In addition, expression of p-Akt proteins in the ovaries from the insulin group was significantly upregulated. Moreover, the Akt inhibitor LY294002 reversed the anti-apoptotic effects of high insulin in the ovary tissues in early pregnancy mice. All of these results showed that insulin impaired ovarian function during early pregnancy and ovarian apoptosis is imbalanced under the role of insulin. The PI3K/AKT signalling pathway might participate in this process.