PROCUREMENT + MAINTENANCE OF GERM-FREE SWINE FOR MICROBIOLOGICAL INVESTIGATIONS
PROCUREMENT + MAINTENANCE OF GERM-FREE SWINE FOR MICROBIOLOGICAL INVESTIGATIONS
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DOI：
10.1128/aem.12.4.295-300.1964
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发表时间：
1964-01-01
期刊：
影响因子：
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通讯作者：
KOHLER, EM
中科院分区：
文献类型：
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作者：
MEYER, RC;BOHL, EH;KOHLER, EM
通过子宫切除术和子宫切开术（剖腹产）常规获得无菌猪。通过轻便的便携式设备，可以获得仔猪并将其运送到超过100英里的距离的实验室（无污染）。饲养中使用的隔离器由不锈钢和柔性塑料膜构成。每隔一周，将粪便拭子和隔离器地板上的废物在血琼脂和巯基乙酸盐肉汤中培养，并在显微镜下检查是否存在细菌、酵母菌和真菌。通过使用富集的PPLO培养基或原代猪肾细胞培养物，未证实此类材料中存在胸膜肺炎样微生物（PPLO）和病毒。专门用于微生物学检查而处死的小猪的组织、体液和盲肠内容物的PPLO、病毒、细菌、酵母菌和真菌也呈阴性。未观察到产前蛔虫感染。与无菌仔猪饲养相关的营养问题，如低血糖，没有遇到，并且使用高压灭菌的商业母猪奶消毒器证明是相当令人满意的。然而，仔猪在出生后最初几天所处的温度是非常重要的。隔离器的设计和应用的gnotobiotic技术采购和饲养的大型无菌动物，如猪证明是可行的，比预期的困难。
Germ-free swine were routinely procured by both hysterectomy and hysterotomy (Caesarian section). By means of light-weight portable equipment, piglets could be obtained and transported to the laboratory (without contamination) over distances in excess of 100 miles. The isolators employed in rearing were constructed of stainless steel and flexible plastic film. At weekly intervals, fecal swabs and waste from the floor of the isolator were cultured on blood-agar and in thioglycolate broth, as well as being examined microscopically for the presence of bacteria, yeast, and fungi. The presence of pleuropneumonia-like organisms (PPLO) and viruses in such material was not demonstrable, either by the use of enriched PPLO media or primary porcine-kidney cell cultures. Tissues, body fluids, and cecal contents of piglets sacrificed specifically for microbiological examination were also negative for PPLO, viruses, bacteria, yeast, and fungi. Prenatal infestations by ascarids were not observed. Nutritional problems related to rearing of germ-free piglets, such as hypoglycemia, were not encountered, and the use of an autoclaved commercial sow''s milk replacer proved quite satisfactory. The temperature to which piglets were subjected during the first few days of life, however, was very important. The isolator design and application of gnotobiotic techniques to the procurement and rearing of a large germ-free animal such as the pig proved feasible and less difficult than anticipated.