Age, sex, and race influence single-strand break repair capacity in a human population.
Age, sex, and race influence single-strand break repair capacity in a human population.
复制标题
年龄、性别和种族影响人群的单链断裂修复能力。
DOI：
10.1016/j.freeradbiomed.2008.08.031
复制
发表时间：
2008
影响因子：
7.4
通讯作者：
Evans,MicheleK
中科院分区：
文献类型：
--
作者：
Trzeciak,AndrzejR;Barnes,Janice;Ejiogu,Ngozi;Foster,Kamala;Brant,LarryJ;Zonderman,AlanB;Evans,MicheleK
Recently, we developed an improved comet assay protocol for evaluating single-strand break repair capacity (SSB-RC) in unstimulated cryopreserved human peripheral blood mononuclear cells (PBMCs). This methodology facilitates control of interexperimental variability [A.R. Trzeciak, J. Barnes, M.K. Evans, A modified alkaline comet assay for measuring DNA repair capacity in human populations. Radiat. Res. 169 (2008) 110–121]. The fast component of SSB repair (F-SSB-RC) was assessed using a novel parameter, the initial rate of DNA repair, and the widely used half-time of DNA repair. The slow component of SSB repair (S-SSB-RC) was estimated using the residual DNA damage after 60 min. We have examined repair of γ-radiation-induced DNA damage in PBMCs from four age-matched groups of male and female whites and African-Americans between ages 30 and 64. There is an increase in F-SSB-RC with age in white females (P<0.01) and nonsignificant decrease in F-SSB-RC in African-American females (P=0.061). F-SSB-RC is lower in white females than in white males (P<0.01). There is a decrease in F-SSB-RC with age in African-American females as compared to white females (P<0.002) and African-American males (nonsignificant, P=0.059). Age, sex, and race had a similar effect on intercellular variability of DNA damage in γ-irradiated and repairing PBMCs. Our findings suggest that age, sex, and race influence SSB-RC as measured by the alkaline comet assay. SSB-RC may be a useful clinical biomarker.
