Simple Method of Synthesizing Nickel-Nitrilotriacetic Acid Gold Nanoparticles with a Narrow Size Distribution for Protein Labeling
Simple Method of Synthesizing Nickel-Nitrilotriacetic Acid Gold Nanoparticles with a Narrow Size Distribution for Protein Labeling
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DOI：
10.1143/jjap.50.095002
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发表时间：
2011-09-01
影响因子：
1.5
通讯作者：
Takahagi, Takayuki
中科院分区：
文献类型：
--
作者：
Kitai, Toshiyuki;Watanabe, Yuta;Takahagi, Takayuki
我们开发了一种简单的方法来合成镍-氮基三乙酸金纳米粒子（Ni-NTA Au NPs），其具有窄的尺寸分布，用于蛋白质复合物的位点特异性标记。采用柠檬酸三钠和单宁酸还原HAuCl（4）制备了Au纳米粒子。然后，用NTA修饰纳米颗粒表面，并随后与Ni（2+）络合。合成的Ni-NTA Au NPs的平均直径为4.3 nm，变异系数为9%。Ni-NTA Au NPs与多组氨酸标记（His标记）的蛋白质的特异性结合通过透射电子显微镜使用驱动蛋白和动力蛋白的p62亚基来确定。因此，我们的方法是有用的分析蛋白质复合物的亚结构。(C)2011日本应用物理学会
We developed a simple method to synthesize nickel-nitrilotriacetic acid gold nanoparticles (Ni-NTA Au NPs) with a narrow size distribution for site-specific labeling in protein complexes. Au NPs were synthesized by the reduction of HAuCl(4) using trisodium citrate and tannin acid. Then, the nanoparticle surfaces were modified with NTA and subsequent complexation with Ni(2+). The mean diameter of the synthesized Ni-NTA Au NPs was 4.3 nm, and the coefficient of variation was 9%. The specific binding of the Ni-NTA Au NPs to polyhistidine-tagged (His-tagged) proteins was determined by transmission electron microscopy using kinesin and the p62 subunit of dynactin. Consequently, our method is useful for analyzing the substructures of protein complexes. (C) 2011 The Japan Society of Applied Physics