A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy
A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy
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使用细胞介电谱评估瞬时受体电位通道活性的新方法
DOI：
10.1016/j.jphs.2020.05.001
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发表时间：
2020
影响因子：
3.5
通讯作者：
Uezono Yasuhito
中科院分区：
文献类型：
--
作者：
Miyano Kanako;Ohbuchi Katsuya;Sudo Yuka;Minami Kouichiro;Yokoyama Toru;Yamamoto Masahiro;Uzu Miaki;Nonaka Miki;Shiraishi Seiji;Murata Hiroaki;Higami Yoshikazu;Uezono Yasuhito
细胞介电谱（CDS）是一种新的技术，使药理学评价多种受体类型的无标记细胞为基础的分析。我们通过使用CDS的基于电阻抗的生物传感器（CellKey™系统）评估了配体门控通道家族、瞬时受体电位香草素1（TRPV 1）和瞬时受体电位锚蛋白1（TRPA 1）通道的活性。用CellKey™测量这些激动剂的效力（EC50）和功效（Emax）与在TRPV 1或TRPA 1表达细胞中使用传统Ca2+流入测定法进行的测量几乎相同，表明CellKey™是评估TRP活性的更简单和更容易的手段。
Cellular dielectric spectroscopy (CDS) is a novel technology enabling pharmacological evaluation of multiple receptor types with a label-free cell-based assay. We evaluated activities of a family of ligand-gated channels, transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) channels by an electrical impedance-based biosensor (CellKey™ system) using CDS. Measures of both potency (EC50) and efficacy (Emax) of these agonists with CellKey™ were almost identical to those made using the traditional Ca2+influx assay in TRPV1- or TRPA1-expressing cells, suggesting that CellKey™ is a simpler and easier means of evaluating TRP activities.