Defects in RNA quality control factors reveal RNAi-independent nucleation of heterochromatin.
Defects in RNA quality control factors reveal RNAi-independent nucleation of heterochromatin.
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DOI：
10.1038/nsmb.2122
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发表时间：
2011-09-04
影响因子：
16.8
通讯作者：
中科院分区：
文献类型：
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Heterochromatin assembly at fission yeast centromeres involves a self-reinforcing loop mechanism wherein chromatin-bound RNAi factors facilitate targeting of Clr4–Rik1 methyltransferase. However, the initial nucleation of heterochromatin has remained elusive. We show that cells lacking Mlo3, a protein involved in mRNP biogenesis and RNA quality control, assemble functional heterochromatin capable of promoting chromosome segregation in RNAi deficient cells. Heterochromatin restoration is linked to RNA surveillance because loss of Mlo3-associated TRAMP also rescues heterochromatin defects of RNAi mutants. Remarkably, mlo3Δ, which causes accumulation of bidirectional repeat-transcripts, restores Rik1 enrichment at repeats, and triggers de novo heterochromatin formation in the absence of RNAi. RNAi-independent heterochromatin nucleation occurs at selected euchromatic loci that show upregulation of antisense RNAs in mlo3Δ cells. We find that the exosome RNA degradation machinery acts parallel to RNAi to promote heterochromatin formation. These results suggest that RNAi-independent mechanisms exploit transcription and non-coding RNAs to nucleate heterochromatin.
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影响因子：
64.5
作者：
Buehler, Marc;Haas, Wilhelm;Moazed, Danesh
通讯作者：
Moazed, Danesh
影响因子：
64.5
作者：
Halic M;Moazed D
通讯作者：
Moazed D
DOI：
10.1073/pnas.232688099
发表时间：
2003-01-07
影响因子：
11.1
作者：
Hall, IM;Noma, K;Grewal, SIS
通讯作者：
Grewal, SIS
DOI：
10.1126/science.1191078
发表时间：
2010-10-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bonasio R;Tu S;Reinberg D
通讯作者：
Reinberg D
DOI：
10.1073/pnas.0813063106
发表时间：
2009-06-02
影响因子：
11.1
作者：
Fischer, Tamas;Cui, Bowen;Grewal, Shiv I. S.
通讯作者：
Grewal, Shiv I. S.