C-type lectins do not act as functional receptors for filovirus entry into cells.
C-type lectins do not act as functional receptors for filovirus entry into cells.
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C型凝集素不作为丝状病毒进入细胞的功能性受体。
DOI：
10.1016/j.bbrc.2010.10.136
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发表时间：
2010
影响因子：
3.1
通讯作者：
Takada,Ayato
中科院分区：
文献类型：
--
作者：
Matsuno,Keita;Nakayama,Eri;Noyori,Osamu;Marzi,Andrea;Ebihara,Hideki;Irimura,Tatsuro;Feldmann,Heinz;Takada,Ayato
据报道，细胞 C 型凝集素通过与丝状病毒糖蛋白 (GP) 上的聚糖结合来促进丝状病毒感染。然而，尚不清楚 C 型凝集素和 GP 之间的相互作用是否介导病毒进入的所有步骤（即附着、内化和膜融合）。在这项研究中，我们生成了用突变型 GP 假型化的水泡性口炎病毒，这些突变型 GP 损害了假定的受体结合区域的结构，从而降低了感染常规用于病毒繁殖的猴肾细胞的能力。我们发现，带有突变 GP 的病毒在表达 C 型凝集素的细胞中的感染力下降，与猴肾细胞中的感染力平行，而这些 GP 与 C 型凝集素的结合活性与感染力的降低无关。这些结果表明，C 型凝集素介导的丝状病毒的进入需要可能参与病毒粒子内化或膜融合的其他细胞分子。
Cellular C-type lectins have been reported to facilitate filovirus infection by binding to glycans on filovirus glycoprotein (GP). However, it is not clearly known whether interaction between C-type lectins and GP mediates all the steps of virus entry (i.e., attachment, internalization, and membrane fusion). In this study, we generated vesicular stomatitis viruses pseudotyped with mutant GPs that have impaired structures of the putative receptor binding regions and thus reduced ability to infect the monkey kidney cells that are routinely used for virus propagation. We found that infectivities of viruses with the mutant GPs dropped in C-type lectin-expressing cells, parallel with those in the monkey kidney cells, whereas binding activities of these GPs to the C-type lectins were not correlated with the reduced infectivities. These results suggest that C-type lectin-mediated entry of filoviruses requires other cellular molecule(s) that may be involved in virion internalization or membrane fusion.