Differential cytokine expression in skin after single and repeated irritation by sodium lauryl sulphate
Differential cytokine expression in skin after single and repeated irritation by sodium lauryl sulphate
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DOI：
10.1111/j.1600-0625.2007.00628.x
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发表时间：
2007-12-01
影响因子：
3.6
通讯作者：
Kezic, Sanja
中科院分区：
文献类型：
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作者：
de Jongh, Cindy M.;Lutter, Rene;Kezic, Sanja
尚不清楚皮肤刺激中细胞因子的体内水平以及中性粒细胞和嗜酸性粒细胞的存在。我们的目的是获得更多的了解炎症介质和标记物参与单次和重复的皮肤刺激。我们使用一种新的技术对表皮来源的液体进行取样，该技术包括在通过激光在角质层中产生微孔后在皮肤上施加负压。在9名志愿者中，在单次4小时10%十二烷基硫酸钠暴露和重复3周暴露（0.1%十二烷基硫酸钠）后对经皮液体进行采样。27种细胞因子通过多重测定法进行评估，IL-1 α、嗜酸性粒细胞阳离子蛋白和髓过氧化物酶通过酶联免疫吸附测定法进行评估。刺激后嗜酸性粒细胞阳离子蛋白水平增加，并与髓过氧化物酶水平相关。炎症介质的水平在未暴露和暴露的皮肤中显示出较大的个体间差异。尽管存在这种变化，但几种介质的水平明显升高：单次和重复暴露后的CC趋化因子配体（CCL）11、CXCL 10和血管内皮生长因子，单次暴露后的IL-1 α和碱性成纤维细胞生长因子以及白细胞介素-1受体拮抗剂（IL-1）重复暴露后RA。与单次暴露相比，重复暴露后CCL 5和IL-1 RA/IL-1 α比值均升高。我们的结论是，单一和重复刺激诱导不同的和协调的各种炎症介质和标志物的表达。
In vivo levels of cytokines and presence of neutrophils and eosinophils in skin irritation are not well known. Our objective was to get more insight in inflammatory mediators and markers involved in single and repeated skin irritation. We sampled epidermis-derived fluid using a novel technology that includes application of a negative pressure on the skin after creation of micropores in the stratum corneum by a laser. In nine volunteers, transdermal fluid was sampled after a single 4-h 10% sodium lauryl sulphate exposure and a repeated 3-week exposure (0.1% sodium lauryl sulphate). Twenty-seven cytokines were assessed by multiplex assay, and IL-1 alpha, eosinophil cationic protein and myeloperoxidase by enzyme-linked immunosorbent assay. Levels of eosinophil cationic protein were increased after irritation and correlated with levels of myeloperoxidase. The levels of inflammatory mediators showed large interindividual differences in unexposed and exposed skin. Despite this variation, several mediators clearly showed increased levels: CC chemokine ligand (CCL)11, CXCL10 and vascular endothelial growth factor after both single and repeated exposure, IL-1 alpha and basic fibroblast growth factor after single exposure and interleukin-1 receptor antagonist (IL-1RA) after repeated exposure. After repeated exposure, CCL5 and the ratio IL-1RA/IL-1 alpha both increased compared with single exposure. We conclude that single and repeated irritation induces differential and concerted expression of various inflammatory mediators and markers.