ON THE STABILITY OF DISPENSABLE CONSTITUENTS OF THE EUKARYOTIC GENOME - STABILITY OF CODING SEQUENCES VERSUS TRULY HYPERVARIABLE SEQUENCES IN A CLONAL VERTEBRATE, THE AMAZON MOLLY, POECILIA-FORMOSA
ON THE STABILITY OF DISPENSABLE CONSTITUENTS OF THE EUKARYOTIC GENOME - STABILITY OF CODING SEQUENCES VERSUS TRULY HYPERVARIABLE SEQUENCES IN A CLONAL VERTEBRATE, THE AMAZON MOLLY, POECILIA-FORMOSA
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DOI：
10.1073/pnas.88.19.8759
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发表时间：
1991-10-01
影响因子：
11.1
通讯作者：
PARZEFALL, J
中科院分区：
文献类型：
--
作者：
SCHARTL, M;SCHLUPP, I;PARZEFALL, J
在无性系单性脊椎动物中，指定雄性的基因变成了无性系。 为了研究这些基因的命运，雌核发育的全雌性鱼福尔摩沙被雄激素处理。 表型男性表现出一套完整的男性特征密切相关的雌雄异株物种，包括身体比例，色素沉着，非常复杂的受精器的poecilid鱼，性行为和精子发生。 这些基因结构的表观稳定性，包括那些参与雄激素调节的基因结构，与非编码序列的高度不稳定性形成对比。 频繁的突变，它们的克隆传播，和至少两个真正的高变基因座导致这些克隆生物体之间的个体差异进行了检测，通过DNA指纹。 这些观察结果证实了这样一个概念，即在“非减数分裂”脊椎动物中，基因组的某些部分比其他部分更容易发生突变。
In clonal unisexual vertebrates, the genes specifying the males become dispensable. To study the fate of such genes the gynogenetic all-female fish Poecilia formosa was treated with androgens. Phenotypic males were obtained that exhibited the complete set of male characteristics of closely related gonochoristic species, including body proportions, pigmentation, the extremely complex insemination apparatus of poeciliid fish, sexual behavior, and spermatogenesis. The apparent stability of such genic structures, including those involved in androgen regulation, is contrasted by high instability of noncoding sequences. Frequent mutations, their clonal transmission, and at least two truly hypervariable loci leading to individual differences between these otherwise clonal organisms were detected by DNA fingerprinting. These observations substantiate the concept that also in "ameiotic" vertebrates certain compartments of the genome are more prone to mutational alterations than others.