Hypoxia facilitates tumour cell detachment by reducing expression of surface adhesion molecules and adhesion to extracellular matrices without loss of cell viability.
Hypoxia facilitates tumour cell detachment by reducing expression of surface adhesion molecules and adhesion to extracellular matrices without loss of cell viability.
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DOI：
10.1038/bjc.1998.299
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发表时间：
1998-06
影响因子：
8.8
通讯作者：
Hart, I R
中科院分区：
文献类型：
--
作者：
Hasan, N M;Adams, G E;Joiner, M C;Marshall, J F;Hart, I R
研究了急性缺氧对两种人黑色素瘤细胞株HMB-2和DX3以及人腺癌细胞株HT29整合素表达和与细胞外基质蛋白黏附的影响。暴露在低氧环境下，细胞表面整合素的表达显著下调，相关的细胞黏附能力下降。细胞黏附和整合素表达的丧失是一过性的，在复氧后24小时内恢复正常。其他细胞黏附分子，如CD44和N-CAM，在细胞暴露于低氧后也下调。细胞汇合处急性暴露于低氧可导致细胞迅速脱离。细胞脱落先于活性丧失。分离的HMB-2和DX3细胞在复氧后完全恢复，漂浮细胞重新附着并继续生长，无论它们是留在原来的玻璃培养皿中还是转移到新的培养容器中，而分离的HT29细胞在复氧后部分恢复。黏附减少后的细胞脱落似乎是一种应激反应，这可能是使恶性细胞逃脱体内缺氧的一个因素，并有可能形成新的肿瘤生长灶。
The effects of acute hypoxia on integrin expression and adhesion to extracellular matrix proteins were investigated in two human melanoma cell lines, HMB-2 and DX3, and a human adenocarcinoma cell line, HT29. Exposure to hypoxia caused a significant down-regulation of cell surface integrins and an associated decrease in cell adhesion. Loss of cell adhesion and integrin expression were transient and levels returned to normal within 24 h of reoxygenation. Other cell adhesion molecules, such as CD44 and N-CAM, were also down-regulated after exposure of cells to hypoxia. Acute exposure to hypoxia of cells at confluence caused rapid cell detachment. Cell detachment preceded loss of viability. Detached HMB-2 and DX3 cells completely recovered upon reoxygenation, and floating cells re-attached and continued to grow irrespective of whether they were left in the original glass dishes or transferred to new culture vessels, while detached HT29 cells partly recovered upon reoxygenation. Cell detachment after decreased adhesion appears to be a stress response, which may be a factor enabling malignant cells to escape hypoxia in vivo, with the potential to form new foci of tumour growth.