Organic composite-mediated surface coating of human acellular bone matrix with strontium
Organic composite-mediated surface coating of human acellular bone matrix with strontium
复制标题
有机复合介导的锶人脱细胞骨基质表面涂层
DOI：
10.1016/j.msec.2017.11.002
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发表时间：
2018-03-01
影响因子：
7.9
通讯作者：
Deng, Li
中科院分区：
文献类型：
--
作者：
Huang, Yi-Zhou;Wang, Jing-Jing;Deng, Li
脱细胞骨基质（ACBM）为骨修复提供了一种骨传导性支架，但其骨诱导性较差。锶（Sr）可提高骨植入物的骨诱导性。在这项研究中，我们开发了一种有机复合物介导的锶涂层ACBM支架的策略，通过使用羧甲基纤维素（CMC）的离子螯合能力和多巴胺（DOPA）的表面粘附能力。在温和条件下合成了CMC-DOPA-Sr复合有机涂层，并对其化学结构和锶离子螯合能力进行了测定。对表面修饰后的锶涂层ACBM（ACBM-Sr）支架进行理化性能表征，并测定其体内外生物相容性和骨诱导性。结果表明，CMC-DOPA-Sr复合材料有利于锶在ACBM支架表面的包覆。ACBM-Sr支架具有锶离子缓释特性，具有良好的细胞相容性，并能促进骨髓间充质干细胞体外成骨分化。此外，ACBM-Sr支架在裸鼠皮下植入后显示出良好的组织相容性。综上所述，本研究提供了一种简单温和的策略，实现锶涂层ACBM支架，这导致了良好的生物相容性和提高骨诱导性。
Acellular bone matrix (ACBM) provides an osteoconductive scaffold for bone repair, but its osteoinductivity is poor. Strontium (Sr) improves the osteoinductivity of bone implants. In this study, we developed an organic composite-mediated strontium coating strategy for ACBM scaffolds by using the ion chelating ability of carboxymethyl cellulose (CMC) and the surface adhesion ability of dopamine (DOPA). The organic coating composite, termed the CMC-DOPA-Sr composite, was synthesized under a mild condition, and its chemical structure and strontium ion chelating ability were then determined. After surface decoration, the physicochemical properties of the strontium-coated ACBM (ACBM-Sr) scaffolds were characterized, and their biocompatibility and osteoinductivity were determined in vitro and in vivo. The results showed that the CMC-DOPA-Sr composite facilitated strontium coating on the surface of ACBM scaffolds. The ACBM-Sr scaffolds possessed a sustained strontium ion release profile, exhibited good cytocompatibility, and enhanced the osteogenic differentiation of mesenchymal stem cells in vitro. Furthermore, the ACBM-Sr scaffolds showed good histocompatibility after subcutaneous implantation in nude mice. Taken together, this study provided a simple and mild strategy to realize strontium coating for ACBM scaffolds, which resulted in good biocompatibility and improved osteoinductivity.