Eukaryotic phytochromes: Light-regulated serine/threonine protein kinases with histidine kinase ancestry
Eukaryotic phytochromes: Light-regulated serine/threonine protein kinases with histidine kinase ancestry
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DOI：
10.1073/pnas.95.23.13976
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发表时间：
1998-11-10
影响因子：
11.1
通讯作者：
Lagarias, JC
中科院分区：
文献类型：
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作者：
Yeh, KC;Lagarias, JC
蓝藻光敏色素组氨酸激酶的发现，以及来自高等植物的光敏色素显示蛋白激酶活性，结合ATP类似物，并具有类似于细菌组氨酸激酶的C-末端结构域的证据。引发了一个有争议的假设，即真核光敏色素家族的光感受器是光调节酶。在这里，我们表明，纯化的重组光敏色素从高等植物和绿色植物表现出丝氨酸/苏氨酸激酶活性类似的光敏色素从黑暗生长的幼苗分离。重组燕麦光敏色素的磷酸化是一个受光和发色团调控的分子内过程。基于比较蛋白质序列比对和生化串扰实验与蓝藻光敏色素Cph 1的反应调节底物，我们提出，真核生物光敏色素是组氨酸激酶旁系同源物与丝氨酸/苏氨酸特异性，其酶活性偏离的原核祖先后，复制的发射模块。
The discovery of cyanobacterial phytochrome histidine kinases, together with the evidence that phytochromes from higher plants display protein kinase activity, bind ATP analogs, and possess C-terminal domains similar to bacterial histidine kinases. has fueled the controversial hypothesis that the eukaryotic phytochrome family of photoreceptors are light-regulated enzymes. Here we demonstrate that purified recombinant phytochromes from a higher plant and a green alga exhibit serine/threonine kinase activity similar to that of phytochrome isolated from dark grown seedlings. Phosphorylation of recombinant oat phytochrome is a light- and chromophore-regulated intramolecular process. Eased on comparative protein sequence alignments and biochemical cross-talk experiments with the response regulator substrate of the cyanobacterial phytochrome Cph1, we propose that eukaryotic phytochromes are histidine kinase paralogs with serine/threonine specificity whose enzymatic activity diverged from that of a prokaryotic ancestor after duplication of the transmitter module.