Metabolic aspects of the role of hyperthermia im mammalian cell inactivation and their possible relevance to cancer treatment.
Metabolic aspects of the role of hyperthermia im mammalian cell inactivation and their possible relevance to cancer treatment.
复制标题
热疗在哺乳动物细胞失活中的作用的代谢方面及其与癌症治疗的可能相关性。
作者：
G. Hahn
摘要 研究了中国仓鼠细胞 (HA1) 代谢状态与细胞对超常热休克 (43°) 敏感性的重要性，并注意到以下结果。 1.细胞对高温的敏感性（以及43°后修复热损伤的能力）与其营养史密切相关。长期缺乏血清（可能还有其他培养基成分）的中国仓鼠细胞变得对热极其敏感。 2.氧气和葡萄糖的作用可能不太重要；慢性或急性缺氧对指数生长或平台期细胞的存活影响很小。对于可用葡萄糖浓度的变化，类似的结论似乎也成立。 3.在热暴露期间或热暴露后不久，在没有血清裂解的情况下，相当一部分细胞被热杀死。血清的存在抑制裂解过程。 4.在41-43°的温度下，细胞对化疗药物、氮芥和博莱霉素敏感，但对亚硝基脲不敏感。这种敏化可能与高温下的修复抑制有关。讨论了这些发现对于可能利用热疗作为常规治疗的辅助手段的重要性。
Summary The importance of the metabolic state of Chinese hamster cells (HA1) in relation to the sensitivity of the cells to supranormal heat shock (43°) was investigated, and the following results were noted. 1.The sensitivity of cells to hyperthermia (as well as their ability to repair heat-induced damage after 43°) is strongly related to their nutritional history. Chinese hamster cells chronically deprived of serum (and probably other medium components) become extremely heat sensitive. 2.The role of oxygen and glucose may be less important; chronic or acute deprivation of oxygen has only minor influence on survival of exponentially growing or plateauphase cells. Similar conclusions appear to be true for variations in available glucose concentrations. 3.An appreciable fraction of cells killed by heat in the absence of serum lyse either during or shortly after heat exposure. The presence of serum inhibits the lysis process. 4.At temperatures of 41–43°, cells are sensitized to the chemotherapeutic agents, nitrogen mustard and bleomycin, but not to a nitrosourea. This sensitization may be related to repair inhibition at the elevated temperatures. The importance of these findings in the possible utilization of hyperthermia as an adjunct to conventional therapy is discussed.