Extraction of Tryptophan enantiomers by aqueous two-phase systems of ethanol and (NH4)2SO4
Extraction of Tryptophan enantiomers by aqueous two-phase systems of ethanol and (NH4)2SO4
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DOI：
10.1002/jctb.4001
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发表时间：
2013-08
影响因子：
3.4
通讯作者：
Xiaoqing Chen;Qiuju Dong;Jingang Yu;F. Jiao
中科院分区：
文献类型：
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作者：
Xiaoqing Chen;Qiuju Dong;Jingang Yu;F. Jiao
背景双水相萃取是一种重要的萃取技术，尤其适用于生物分离。本工作的重点是使用一种新型的水两相系统（ATPS），色氨酸的对映体分离。结果只有少数几种盐能与乙醇形成有效相。考察了不同盐类的盐析能力和ATPS的最终特性。结果表明：增加相组分的数量和降低pH从6到2.5都有利于色氨酸的对映体分离；对映选择性萃取和分配行为主要取决于溶液的pH、手性萃取剂的种类和浓度。此外，盐、乙醇和温度对对映体分离也有一定的影响。最后提出了色氨酸对映体的最合理提取机理。结论本研究提出的乙醇/硫酸铵组成的新型ATPS具有较好的色氨酸对映体提取能力。©2012化工学会
Background As an important extraction technique, aqueous two-phase extraction is especially applicable to bioseparation. This work has focused on using a novel kind of aqueous two-phase system (ATPS), for the enantioseparation of tryptophan. Results Only limited kinds of salts could form effective phases with ethanol. The salting-out abilities of various salts and the final characteristics of ATPS were investigated. The results show that increase in the amount of phase-forming components and decrease in pH from 6 to 2.5 are both beneficial for the enantioseparation of tryptophan; enantioselective extraction and partitioning behavior are mainly dependent on the pH of solution, the type of chiral extractant and its concentration. Furthermore, salt, ethanol and temperature also have some effects on the enantioseparation. Finally, the most reasonable extraction mechanism of the tryptophan enantiomers was suggested. Conclusion The novel ATPS composed of ethanol/ammonium sulfate proposed in this studyhas good ability for the extraction of tryptophan enantiomers. © 2012 Society of Chemical Industry