RNA14 AND RNA15 PROTEINS AS COMPONENTS OF A YEAST PRE-MESSENGER-RNA 3'-END PROCESSING FACTOR
RNA14 AND RNA15 PROTEINS AS COMPONENTS OF A YEAST PRE-MESSENGER-RNA 3'-END PROCESSING FACTOR
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DOI：
10.1126/science.7992054
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发表时间：
1994-12-09
期刊：
影响因子：
56.9
通讯作者：
KELLER, W
中科院分区：
文献类型：
--
作者：
MINVIELLESEBASTIA, L;PREKER, PJ;KELLER, W
大多数真核细胞的前信使RNA在其3‘端通过内切和多聚腺苷酸化被加工。在酵母中，这一过程需要多聚腺苷[聚(A)]聚合酶(PAP)和其他尚未被表征的蛋白质。在这里，PAP1基因的突变被证明与先前发现的RNA14和RNA15基因的突变具有协同致命性，这表明它们的编码蛋白参与了3‘端的加工。事实上，ma14和ma15突变体的提取物在这两个加工步骤中都显示出缺陷。生化互补实验和用部分纯化的裂解因子I(CFI)重组这两种活性证实了遗传预测。
Most eukaryotic pre-messenger RNAs are processed at their 3' ends by endonucleolytic cleavage and polyadenylation. In yeast, this processing requires polyadenylate [poly(A)] polymerase (PAP) and other proteins that have not yet been characterized. Here, mutations in the PAP1 gene were shown to be synergistically lethal with previously identified mutations in the RNA14 and RNA15 genes, which suggests that their encoded proteins participate in 3'-end processing. Indeed, extracts from ma14 and ma15 mutants were shown to be deficient in both steps of processing. Biochemical complementation experiments and reconstitution of both activities with partially purified cleavage factor I (CF I) validated the genetic prediction.