Cloning and characterization of the SnRK2 gene family from Zea mays
Cloning and characterization of the SnRK2 gene family from Zea mays
复制标题
玉米 SnRK2 基因家族的克隆和表征
DOI：
10.1007/s00299-008-0608-8
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发表时间：
2008-12-01
影响因子：
6.2
通讯作者：
Wang, Guoying
中科院分区：
文献类型：
--
作者：
Huai, Junling;Wang, Meng;Wang, Guoying
SnRK 2基因家族是一组植物特异性蛋白激酶，与阿坝和非生物胁迫信号传导有关。我们在玉米中发现了11个SnRK 2，命名为ZmSnRK2.1至ZmSnRK2.11，并克隆了其中的10个。通过分析拟南芥、水稻和玉米中所有SnRK 2的基因结构，我们发现大多数SnRK 2的7个外显子长度保守。虽然C-末端是不同的，我们发现七个保守的基序。其中，基序1是所有SnRK 2基因共有的。基于激酶结构域和基序1的系统发育分析，SnRK 2被分为三组。基序4和基序5在第一组中特异存在，这一组中的许多基因已被证实受阿坝诱导。这一结果表明，这两个基序介导的阿坝反应。利用实时荧光定量PCR技术对ZmSnRK 2基因的表达模式进行了分析，结果表明ZmSnRK 2基因受一种或多种非生物胁迫诱导表达，可能在玉米逆境响应中发挥重要作用。
The SnRK2 gene family is a group of plant-specific protein kinases that has been implicated in ABA and abiotic stress signaling. We found 11 SnRK2s in maize, assigned names from ZmSnRK2.1 to ZmSnRK2.11 and cloned ten of them. By analyzing the gene structure of all the SnRK2s from Arabidopsis, rice, and maize, we found seven exons that were conserved in length among most of the SnRK2s. Although the C-terminus was divergent, we found seven conserved motifs. Of these, motif 1 was common to all of the SnRK2 genes. Based on phylogenetic analysis using the kinase domain and motif 1, the SnRK2s were divided into three groups. Motifs 4 and 5 were found specifically in group I, and many genes of this group have been confirmed to be induced by ABA. This result suggests that these two motifs mediate the ABA response. The expression patterns of ZmSnRK2 genes were characterized by using quantitative real-time RCR, which revealed that ZmSnRK2 genes were induced by one or more abiotic stress treatments and therefore may play important roles in maize stress responses.