THE STOICHIOMETRY OF THE EXCHANGE CATALYZED BY THE MITOCHONDRIAL CALCIUM SODIUM ANTIPORTER
THE STOICHIOMETRY OF THE EXCHANGE CATALYZED BY THE MITOCHONDRIAL CALCIUM SODIUM ANTIPORTER
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DOI：
10.1042/bj2290161
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发表时间：
1985-01-01
影响因子：
4.1
通讯作者：
BRAND, MD
中科院分区：
文献类型：
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作者：
BRAND, MD
大鼠心脏线粒体呼吸琥珀酸(以抑制对钙离子单一转运体的摄取)，在钙/钠逆向转运体上释放钙，直到达到稳定状态。添加离子载体A23187(催化Ca~(2+)/2H~+交换)并没有扰乱这种稳定状态。热力学分析表明，当钙离子/NNA+交换处于平衡状态时，加入A23187会引起线粒体外游离[Ca~(2+)]的明显变化。线粒体内源性Ca/Na逆向转运蛋白催化电子中性型钙/2Na+交换。
Rat heart mitochondria respiring on succinate in the presence of Ru red (to inhibit uptake on the Ca2+ uniporter) released Ca2+ on the Ca/Na antiporter until a steady state was reached. Addition of the ionophore A23187 (which catalyzes Ca2+/2H+ exchange) did not perturb this steady state. Thermodynamic analysis showed that if a Ca2+/nNa+ exchange with any value of n was at equilibrium, addition of A23187 would cause an obvious change in extramitochondrial free [Ca2+]. The endogenous Ca/Na antiporter of mitochondria catalyses electroneutral Ca2+/2Na+ exchange.