Two novel nuclear genes, OsSIG5 and OsSIG6, encoding potential plastid sigma factors of RNA polymerase in rice:: Tissue-specific and light-responsive gene expression
Two novel nuclear genes, OsSIG5 and OsSIG6, encoding potential plastid sigma factors of RNA polymerase in rice:: Tissue-specific and light-responsive gene expression
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DOI：
10.1093/pcp/pcl050
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发表时间：
2007-01-01
影响因子：
4.9
通讯作者：
Asayama, Munehiko
中科院分区：
文献类型：
--
作者：
Kubota, Yoshiki;Miyao, Akio;Asayama, Munehiko
OsSIG 5和OsSIG 6是两个新的核基因，编码水稻质体σ RNA聚合酶（RNAP）。推导的氨基酸序列包含保守区域，区域1.2-4.2，和在N-末端的新区域A/B。OsSIG 5和OsSIG 6的组织特异性和光响应性转录本。OsSig 5的N-末端区域赋予绿色荧光蛋白到叶绿体的输入。利用重组OsSig 5-RNAP全酶体外合成水稻psbA特异性转录本。这些结果表明OsSig 5是质体σ因子。这是第一次在作物中报道Sig 5型sigma因子。
Two novel nuclear genes, OsSIG5 and OsSIG6, encoding potential plastid sigma factors of RNA polymerase (RNAP) were identified in Oryza sativa. The deduced amino acid sequences contain conserved regions, regions 1.2-4.2, and a novel region A/B at the N-terminus. Tissue-specific and light-responsive transcripts of OsSIG5 and OsSIG6 were observed. The N-terminal region of OsSig5 conferred import of green fluorescent protein into the chloroplast. Specific transcripts of rice psbA were synthesized in vitro by reconstituted OsSig5-RNAP holoenzymes. These results indicated that OsSig5 is a plastid sigma factor. This is the first report of the Sig5-type sigma factor in crops.